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NBS1抗体 [1C3]

KO/KD Orthogonal
Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222
Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222
Anti-NBS1 antibody [1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70222
Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222
Anti-NBS1 antibody [1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70222
Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222

目录号 GTX70222

文献引用 15

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 1C3

同种型

 IgG1

实验应用

 WB, ICC/IF, IP

种属反应

 Human, Mouse
产品包装
100 μl (¥4300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

85 kDa. ( Note )

阳性对照

293T , MCF7 , HeLa , Raji

产品属性

形式

Liquid

存储溶液

1XPBS (pH7), 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

The complete coding region of the human p95/NBS1 expressed in E. coli.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_372443

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

nibrin , AT-V1 , AT-V2 , ATV , NBS , NBS1 , P95

细胞定位

Nucleus

功能与背景

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222

GTX70222 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1C3] (GTX70222) diluted at 1:500.

Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222

GTX70222 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX70222 diluted at 1:1000

Anti-NBS1 antibody [1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70222

GTX70222 ICC/IF Image

Immunofluorescence analysis of HeLa, using NBS1(GTX70222) antibody at 1:100 dilution.

Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222

GTX70222 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1C3] (GTX70222) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NBS1 antibody [1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70222

GTX70222 ICC/IF Image

Orthogonal

The data was published in the journal J Virol in 2017.PMID: 28855246

Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222

GTX70222 WB Image

The data was published in the journal BMC Biol in 2015. PMID: 25857663

文献引用

Application Reference

Tomioka K et al. Sci Rep 2021; 11 (1) : 19661 NBS1 I171V variant underlies individual differences in chromosomal radiosensitivity within human populations.
实验应用 : WB
Hollingworth R et al. J Gen Virol 2020; 101 (8) : 873-883 Productive herpesvirus lytic replication in primary effusion lymphoma cells requires S-phase entry.
实验应用 : WB
种属反应 : Human
Qi F et al. J Radiat Res 2020; 61 (3) : 368-375 FXR1 is a novel MRE11-binding partner and participates in oxidative stress responses.
实验应用 : WB
种属反应 : Human
Ly P et al. Nat Genet 2019; 51 (4) : 705-715 Chromosome segregation errors generate a diverse spectrum of simple and complex genomic rearrangements.
Raghunandan M et al. Hum Mol Genet 2019; (Epub) Functional crosstalk between the Fanconi anemia and ATRX/DAXX histone chaperone pathways promotes replication fork recovery.
实验应用 : WB
种属反应 : Human
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Application Reference
Tomioka K et al. Sci Rep 2021; 11 (1):19661 NBS1 I171V variant underlies individual differences in chromosomal radiosensitivity within human populations.
实验应用 : WB
Hollingworth R et al. J Gen Virol 2020; 101 (8):873-883 Productive herpesvirus lytic replication in primary effusion lymphoma cells requires S-phase entry.
实验应用 : WB
种属反应 : Human
Qi F et al. J Radiat Res 2020; 61 (3):368-375 FXR1 is a novel MRE11-binding partner and participates in oxidative stress responses.
实验应用 : WB
种属反应 : Human
Ly P et al. Nat Genet 2019; 51 (4):705-715 Chromosome segregation errors generate a diverse spectrum of simple and complex genomic rearrangements.
Raghunandan M et al. Hum Mol Genet 2019; (Epub) Functional crosstalk between the Fanconi anemia and ATRX/DAXX histone chaperone pathways promotes replication fork recovery.
实验应用 : WB
种属反应 : Human
Hollingworth R et al. J Virol 2017; 91 (22) Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus.
Petroni M et al. Cell Death Differ 2015; The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress.
实验应用 : WB
种属反应 : Human
Harten SK et al. BMC Biol 2015; 13:21 The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
实验应用 : WB
种属反应 : Human
Cesare AJ et al. Mol Cell 2013; 51 (2):141-55 The telomere deprotection response is functionally distinct from the genomic DNA damage response.
实验应用 : WB
种属反应 : Human
Anderson VE et al. Cancer Res 2011; 71 (2):463-72 CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors.
Suzuki K et al. Radiat Prot Dosimetry 2011; 143 (43135):279-83 ATM-dependent cellular response to DNA double strand breaks plays a pivotal role in the maintenance of the integrity of the genome.
Chailleux C et al. Biochem J 2010; 426 (3):365-71 Physical interaction between the histone acetyl transferase Tip60 and the DNA double-strand breaks sensor MRN complex.
实验应用 : WB
种属反应 : Human
Tseng SF et al. J Biol Chem 2005; 280 (47):39594-600 Importin KPNA2 is required for proper nuclear localization and multiple functions of NBS1.
实验应用 : ICC/IF
种属反应 : Human
Giannini G et al. Oncogene 2004; 23 (15):270463 Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability.
实验应用 : ICC/IF
种属反应 : Human
Xu X et al. FASEB J 2003; 17 (13):1842-8 NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors.
实验应用 : ICC/IF
种属反应 : Human
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4300
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