Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.

NBS1 antibody immunoprecipitates nibrin protein in IP experiments.
IP samples: NT2D1 whole cell extract
A. 50 μg NT2D1 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of nibrin protein by 4 μg NBS1 antibody (GTX103229)
7.5 % SDS-PAGE
The immunoprecipitated nibrin protein was detected by NBS1 antibody (GTX103229) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

NBS1 antibody detects NBS1 protein by western blot analysis.
A. 30 μg NT2D1 whole cell extract
7.5 % SDS-PAGE
NBS1 antibody (GTX103229) dilution: 1:5000

NBS1 antibody detects NBS1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NBS1 stained by NBS1 antibody (GTX103229) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.