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NDP52抗体 [GT422]

KO/KD Orthogonal
Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396
Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396
Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396
Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396
Anti-NDP52 antibody [GT422] used in Immunoprecipitation (IP). GTX630396
Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396
Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396
Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396
Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396

目录号 GTX630396

文献引用 2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT422

同种型

 IgG1

实验应用

 WB, ICC/IF, IP

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 NDP52 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

52 kDa. ( Note )

阳性对照

Jurkat , Raji , NCI-H929 , Hep3B , Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr) , HepG2 (3 μM Thapsigargin treatment for 12 hr)

预测种属反应

Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888207

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

calcium binding and coiled-coil domain 2 , NDP52

细胞定位

Cytoplasm , perinuclear region , Golgi apparatus , cytoskeleton

功能与背景

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

Orthogonal

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396

GTX630396 ICC/IF Image

Orthogonal

NDP52 antibody [GT422] detects NDP52 protein at autophagosome  by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

Orthogonal

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
A. 30 μg Huh7 whole cell lysate/extract
B. 30 μg Hep3B whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT422] (GTX630396) dilution: 1:1000

Anti-NDP52 antibody [GT422] used in Immunoprecipitation (IP). GTX630396

GTX630396 IP Image

Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody [GT422] (GTX630396).
Western blot analysis was performed using NDP52 antibody [GT422] (GTX630396).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396

GTX630396 ICC/IF Image

NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec.
Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT422] (GTX630396) dilution: 1:1000

Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396

GTX630396 ICC/IF Image

NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

文献引用

Application Reference

Yeo SK et al. Autophagy 2024; 20 (3) : 525-540 AZI2 mediates TBK1 activation at unresolved selective autophagy cargo receptor complexes with implications for CD8 T-cell infiltration in breast cancer.
Prabakaran T et al. EMBO J 2018; (Epub) Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1.
实验应用 : WB
种属反应 : Human
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产品包装 报价 (¥)
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¥ 1700
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