NDP52抗体

Name: NDP52抗体
Brand: GeneTex
SKU: GTX115378
Price: 4000.00 RMB
Rating: 4 (1 reviews)

Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

Anti-NDP52 antibody used in Immunoprecipitation (IP). GTX115378

Anti-NDP52 antibody used in IHC (Paraffin sections) (IHC-P). GTX115378

目录号 GTX115378

文献引用 13

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP
种属反应	Human
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 NDP52 的产品

View product citations for antibody GTX115378 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

52 kDa. ( Note )

阳性对照

Raji , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr) , Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr)

预测种属反应

Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.57 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10620266

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

calcium binding and coiled-coil domain 2 , NDP52

细胞定位

Cytoplasm , perinuclear region , Golgi apparatus , cytoskeleton

功能与背景

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

数据库

Gene ID: 10241 CALCOCO2
UniProt: Q13137 CALCOCO2

研究领域

產品图片

GTX115378 ICC/IF Image

NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 stained by NDP52 antibody (GTX115378) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

GTX115378 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115378 IP Image

Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody (GTX115378).
Western blot analysis was performed using NDP52 antibody (GTX115378).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX115378 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115378 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NDP52(GTX115378) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115378 WB Image

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115378 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX115378 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115378 WB Image

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115378 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 29187741

GTX115378 ICC/IF Image

The data was published in the journal Nat Commun in 2017. PMID: 29187741

GTX115378 ICC/IF Image

The data was published in the journal Dev Cell in 2019.PMID: 31353311

文献引用

Application Reference

Dos Santos ? et al. Nat Commun 2023; 14 (1) : 2855 Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription.

Shiho Fukushima et al. Front Cell Infect Microbiol 2022; 12 : 829682 Recruitment of LC3 by Campylobacter jejuni to Bacterial Invasion Site on Host Cells via the Rac1-Mediated Signaling Pathway.

实验应用 : WB

种属反应 : Human

Po-Yuan Ke et al. Cells 2022; 11 (7) Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN.

种属反应 : Human

Sabrina Robichaud et al. Autophagy 2021; 17 (11) : 3671-3689 Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells.

实验应用 : ICC/IF

种属反应 : Human

Hall BS et al. Autophagy 2021; : 1-19 Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity.

实验应用 : ICC/IF

种属反应 : Human

显示更多 +

我要回报文献

产品评论

NDP52 antibody 目录号 GTX115378

Rating

( Average 4 based on 1 users reviews)

实验应用

Immunocytochemistry/ Immunofluorescence(ICC/IF)

( Average 4 based on 1 users reviews)

Date : Anonymous submitted on 03-May-2017

Score :

Application Tested : ICC/IF

Sample Species : Hu

Sample : HeLa cells

Blocking : 5% BSA+10 mM glycine in PBS, 25°C, 1Hr

Fixation : 4% Paraformaldehyde in PBS

Permeabilization /Antigen retrieval : 0.1% Triton X in PBS

Primary Antibody : 1:500, 4°C, 16Hr

我要评论产品

Application Reference

Dos Santos ? et al. Nat Commun 2023; 14 (1):2855 Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription.

Shiho Fukushima et al. Front Cell Infect Microbiol 2022; 12:829682 Recruitment of LC3 by Campylobacter jejuni to Bacterial Invasion Site on Host Cells via the Rac1-Mediated Signaling Pathway.

实验应用 : WB

种属反应 : Human

Po-Yuan Ke et al. Cells 2022; 11 (7) Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN.

种属反应 : Human

Sabrina Robichaud et al. Autophagy 2021; 17 (11):3671-3689 Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells.

实验应用 : ICC/IF

种属反应 : Human

Hall BS et al. Autophagy 2021;:1-19 Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity.

实验应用 : ICC/IF

种属反应 : Human

Hung CM et al. Sci Adv 2021; 7 (15) AMPK/ULK1-mediated phosphorylation of Parkin ACT domain mediates an early step in mitophagy.

实验应用 : ICC/IF

Ogawa M et al. Commun Biol 2020; 3 (1):25 Streptococcus pneumoniae triggers hierarchical autophagy through reprogramming of LAPosome-like vesicles via NDP52-delocalization.

Wang X et al. Biomed Pharmacother 2020; 127:110125 Mechanisms underlying astrocytic connexin-43 autophagy degradation during cerebral ischemia injury and the effect on neuroinflammation and cell apoptosis.

实验应用 : WB

Zachari M et al. Dev Cell 2019; 50 (5):627-643.e5 Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.

实验应用 : ICC/IF

种属反应 : Human

Liao CC et al. Autophagy 2018; 14 (12):2065-2082 Autophagic degradation of SQSTM1 inhibits ovarian cancer motility by decreasing DICER1 and AGO2 to induce MIRLET7A-3P.

Fili N et al. Nat Commun 2017; 8 (1):1871 NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription.

实验应用 : WB, IP

种属反应 : Human

Chen CH et al. Clin Cancer Res 2017; (Epub) Dual Inhibition of PIK3C3 and FGFR as a New Therapeutic Approach to Treat Bladder Cancer.

实验应用 : WB

Kimura T et al. Acta Neuropathol Commun 2017; 5 (1):67 Age-dependent changes in synaptic plasticity enhance tau oligomerization in the mouse hippocampus.

实验应用 : WB

SDS
Glycerol.pdf
Thimerosal.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

	(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
	http://www.neobioscience.com
	genetex@neobioscience.com

NDP52抗体

目录号 GTX115378

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX115378 ICC/IF Image

GTX115378 WB Image

GTX115378 IP Image

GTX115378 WB Image

GTX115378 IHC-P Image

GTX115378 WB Image

GTX115378 WB Image

GTX115378 WB Image

GTX115378 WB Image

GTX115378 ICC/IF Image

GTX115378 ICC/IF Image

文献引用

Application Reference

产品评论

NDP52 antibody 目录号 GTX115378

产品

研究

支持

公司

Follow Us

粤ICP备08007346号-9

© 1998-2022 GeneTex, Inc. 保留所有权利。

所有产品仅供研究使用 - 不应用于诊断或治疗。