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NF-H抗体

Anti-NF-H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110065
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065
Anti-NF-H antibody used in IHC (Paraffin sections) (IHC-P). GTX110065
Anti-NF-H antibody used in IHC (Paraffin sections) (IHC-P). GTX110065
Anti-NF-H antibody used in Western Blot (WB). GTX110065
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065
Anti-NF-H antibody used in Western Blot (WB). GTX110065

目录号 GTX110065

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

112 kDa. ( Note )

阳性对照

rat brain , IMR32

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human NF-H. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950985

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

neurofilament heavy , CMT2CC , NFH

功能与背景

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-NF-H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110065

GTX110065 ICC/IF Image

NF-H antibody detects NF-H protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065

GTX110065 IHC-Fr Image

NF-H antibody detects NF-H protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065

GTX110065 IHC-Fr Image

NF-H antibody detects NF-H protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in IHC (Paraffin sections) (IHC-P). GTX110065

GTX110065 IHC-P Image

NF-H antibody detects NF-H protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in IHC (Paraffin sections) (IHC-P). GTX110065

GTX110065 IHC-P Image

NF-H antibody detects NF-H protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
NF-H stained by NF-H antibody (GTX110065) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-H antibody used in Western Blot (WB). GTX110065

GTX110065 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody (GTX110065) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065

GTX110065 IHC-Fr Image

NF-H antibody detects NF-H protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in Western Blot (WB). GTX110065

GTX110065 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody (GTX110065) diluted at 1:500.

文献引用

Application Reference

Treffy RW et al. Dev Cell 2021; 56 (19) : 2752-2764.e6 Neuroblastoma differentiation in?vivo excludes cranial tumors.
Tomita Y et al. Biochem Biophys Res Commun 2019; 519 (3) : 462-468 Ninjurin 1 mediates peripheral nerve regeneration through Schwann cell maturation of NG2-positive cells.
Pigna E et al. EBioMedicine 2019; 40 : 717-732 Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosis.
实验应用 : IHC-Fr
种属反应 : Mouse
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Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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