产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLa , HepG2 , NIH-3T3 , A431
产品说明
GTX101150 specifically recognizes NFkB p100 isoform, but not the cleaved p52 isoform.
预测种属反应
Rat, Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human NFkB p100. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1241105
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
nuclear factor kappa B subunit 2 , CVID10 , H2TF1 , LYT-10 , LYT10 , NF-kB2 , p100 , p49/p100 , p52
细胞定位
Nucleus , Cytoplasm
功能与背景
NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM]
数据库
研究领域
產品图片
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/templates/default/js/lightbox/images/loading.gif "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
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GTX101150 WB Image
NFkB p100 antibody [C2C3], C-term detects NFkB p100 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:1000.
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GTX101150 IP Image
NFkB p100/p52 antibody immunoprecipitates NFkB p100/p52 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. 30 μg Jurkat whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p100/p52 protein by 2 μg of NFkB p100/p52 antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated NFkB p100/p52 protein was detected by NFkB p100/p52 antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101150 WB Image
Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101150 WB Image
Sample (30 ug of whole cell lysate)
A:NIH-3T3 7.5% SDS PAGE
GTX101150 diluted at 1:1000
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GTX101150 WB Image
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
7.5% SDS PAGE
NFkB p100 antibody
GTX101150 diluted at 1:1000
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文献引用
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产品评论
NFkB p100 antibody [C2C3], C-term
目录号 GTX101150
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Rating
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( Average 4 based on 1 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 09-Apr-2013
Application Tested :
WB
Sample Species :
Hu
Sample :
YY-8103 cells
Amount used :
30μg
Blocking :
5% non-fat milk, 25°C, 0.5Hr
Primary Antibody :
1:500, 25°C, 2Hr
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/upload/website/prouct_img/small/GTX101150/GTX101150_39549_20150924_WB_w_23060100_654.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101150](/upload/website/prouct_img/small/GTX101150/GTX101150_39549_IP_w_23060100_847.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/upload/website/prouct_img/small/GTX101150/GTX101150_39549_20170427_WB_KO_watermark_w_23060100_524.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/upload/website/prouct_img/small/GTX101150/GTX101150_WB_M_w_23060100_829.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/upload/website/prouct_img/small/GTX101150/GTX101150_WB_w_23060100_395.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/upload/website/prouct_img/normal/GTX101150/GTX101150_39549_20150924_WB_w_23060100_654.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101150](/upload/website/prouct_img/normal/GTX101150/GTX101150_39549_IP_w_23060100_847.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/upload/website/prouct_img/normal/GTX101150/GTX101150_39549_20170427_WB_KO_watermark_w_23060100_524.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/upload/website/prouct_img/normal/GTX101150/GTX101150_WB_M_w_23060100_829.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/upload/website/prouct_img/normal/GTX101150/GTX101150_WB_w_23060100_395.webp "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
![Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150](/upload/website/support/rewardsprogram/normal/genetex_GTX101150_WB_19080419_ba0e89bb-8bd3-45a4-8a4f-ea5c214134995442.jpg "Anti-NFkB p100 antibody [C2C3], C-term used in Western Blot (WB). GTX101150")
