产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
A431 , HeLa
预测种属反应
Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human NFkB p105. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950995
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50
细胞定位
Nucleus , Cytoplasm
功能与背景
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX110585 ChIP assay Image
NFkB p105 antibody immunoprecipitates NFkB p105 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of NFkB p105 antibody (GTX110585) The precipitated DNA was detected by PCR with primer set targeting to PPM1D promoter.
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GTX110585 WB Image
Wild-type (WT) and NFkB p105 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p105 antibody (GTX110585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110585 IHC-P Image
NFkB p105 antibody detects secreted NFkB p105 protein by immunohistochemical analysis.Sample: Paraffin-embedded dog colon.NFkB p105 stained by NFkB p105 antibody (GTX110585) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110585 IHC-P Image
NFkB p105 antibody detects NFkB p105 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog kidney.NFkB p105 stained by NFkB p105 antibody (GTX110585) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110585 IHC-P Image
NFkB p105 antibody detects NFkB p105 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog intestine.NFkB p105 stained by NFkB p105 antibody (GTX110585) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110585 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p105 antibody (GTX110585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110585 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NFkB p105 (GTX110585) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110585 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFkB p105(GTX110585) antibody at 1:200 dilution.
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GTX110585 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX110585 diluted at 1:1000
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文献引用
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