产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:500-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
GFP-human NF-kappa-B-transfected 293T , Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2
产品说明
IP/MS validation was supported by references (PMID:30377388)
预测种属反应
Bovine, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.37 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1951681
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65
细胞定位
Nucleus , Cytoplasm
功能与背景
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
数据库
研究领域
產品图片
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GTX107678 IP Image
NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 μg of NFkB p65 antibody (GTX107678) 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody (GTX107678) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX107678 WB Image
Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX107678 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000 and competitor's antibody (#4764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX107678 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX107678 ICC/IF Image
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFkB p65 protein stained by NFkB p65 antibody (GTX107678) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
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GTX107678 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(GTX107678) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX107678 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX107678 IHC-P Image
NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107678 IHC-P Image
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107678 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX107678 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX107678 IHC-P Image
NFkB p65 antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107678 WB Image
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX107678 IHC-P Image
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse cervix.NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107678 IHC-P Image
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107678 ICC/IF Image
NFkB p65 antibody detects NFkB p65 protein at cytoplasm, nucleus and endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NFkB p65 stained by NFkB p65 antibody (GTX107678) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX107678 WB Image
The data was published in the journal Evid Based Complement Alternat Med in 2014. PMID: 24723958
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GTX107678 WB Image
The data was published in the 2021 in Transl Neurosci. PMID: 33708438
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GTX107678 WB Image
The data was published in the journal Nat Commun in 2018.PMID: 29968710
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文献引用
产品评论
NFkB p65 antibody
目录号 GTX107678
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Rating
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( Average 4.7 based on 3 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4.7 based on 3 users reviews)
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Date :
Anonymous submitted on 23-Jun-2020
Application Tested :
WB
Sample Species :
Ms
Sample :
RAW264.7 cells
Amount used :
10 μg
Blocking :
0.5% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:5000, 25°C, 12Hr
Date :
Anonymous submitted on 08-Jan-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
PANC1 cells
Amount used :
25 μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:500, 4°C, 16Hr
Date :
Anonymous submitted on 15-Mar-2017
Application Tested :
WB
Sample Species :
Hu
Sample :
SAS cells
Amount used :
15 μg
Blocking :
3% BSA in TBST, 25°C, 1Hr
Primary Antibody :
1:500, 4°C, 16Hr
