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NFkB p65 (phospho Ser536)抗体

Orthogonal
Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899
Anti-NFkB p65 (phospho Ser536) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133899
Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899
Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899

目录号 GTX133899

文献引用 9
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 NFkB p65 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000
ICC/IF Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

60 kDa. ( Note )

预测种属反应

Bovine, Pig, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide surrounding phospho Ser536 of human NFkB p65. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2887135

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

细胞定位

Nucleus,Cytoplasm

功能与背景

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

数据库

研究领域

產品图片

Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899

GTX133899 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 (phospho Ser536) antibody (GTX133899) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 (phospho Ser536) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133899

GTX133899 ICC/IF Image

NFkB p65 (phospho Ser536) antibody detects NFkB p65 (phospho Ser536) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFkB p65 (phospho Ser536) stained by NFkB p65 (phospho Ser536) antibody (GTX133899) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899

GTX133899 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 (phospho Ser536) antibody (GTX133899) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899

GTX133899 WB Image

Orthogonal

The data was published in the 2022 in Front Pharmacol. PMID: 35308231

文献引用

Application Reference

Li D et al. Heliyon 2024; 10 (5) : e27644 DL-3-n-butylphthalide attenuates doxorubicin-induced acute cardiotoxicity via Nrf2/HO-1 signaling pathway.
Li CJ et al. J Hepatocell Carcinoma 2023; 10 : 123-138
实验应用 : WB
种属反应 : Human
Zhang W et al. Oxid Med Cell Longev 2023; 2023 : 9966355 Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3.
实验应用 : WB
种属反应 : Mouse, Rat
Alhasan H et al. Nat Commun 2022; 13 (1) : 3919 Inhibitory role of Annexin A1 in pathological bone resorption and therapeutic implications in periprosthetic osteolysis.
实验应用 : WB
种属反应 : Human
Chou LY et al. Cells 2022; 11 (20) Paracrine Senescence of Mesenchymal Stromal Cells Involves Inflammatory Cytokines and the NF-κB Pathway.
实验应用 : WB
种属反应 : Human
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产品评论

NFkB p65 (phospho Ser536) antibody 目录号 GTX133899

Rating
( Average 4 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-NFkB p65 (phospho Ser536) antibody used in Western Blot (WB). GTX133899
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : ARPE19 cell lysates
Amount used : 10μg
Blocking : 5% non-fat milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 16°C, 4Hr
我要评论产品
Application Reference
Li D et al. Heliyon 2024; 10 (5):e27644 DL-3-n-butylphthalide attenuates doxorubicin-induced acute cardiotoxicity via Nrf2/HO-1 signaling pathway.
Li CJ et al. J Hepatocell Carcinoma 2023; 10:123-138
实验应用 : WB
种属反应 : Human
Zhang W et al. Oxid Med Cell Longev 2023; 2023:9966355 Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3.
实验应用 : WB
种属反应 : Mouse, Rat
Li Y et al. Exp Ther Med 2022; 24 (3):567 Interleukin 32 participates in cardiomyocyte-induced oxidative stress, inflammation and apoptosis during hypoxia/reoxygenation via the NOD2/NOX2/MAPK signaling pathway.
实验应用 : WB
种属反应 : Human
Alhasan H et al. Nat Commun 2022; 13 (1):3919 Inhibitory role of Annexin A1 in pathological bone resorption and therapeutic implications in periprosthetic osteolysis.
实验应用 : WB
种属反应 : Human
Chou LY et al. Cells 2022; 11 (20) Paracrine Senescence of Mesenchymal Stromal Cells Involves Inflammatory Cytokines and the NF-κB Pathway.
实验应用 : WB
种属反应 : Human
Xue Zhang et al. Front Pharmacol 2022; 13:827128 A Polysaccharide From Eupolyphaga sinensis Walker With Anti-HBV Activities In Vitro and In Vivo.
实验应用 : WB
种属反应 : Human
Chang JW et al. Cereb Cortex 2020; 30 (8):4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
实验应用 : WB
种属反应 : Rat
Chao CC et al. Cancers (Basel) 2020; 12 (2) CXCL1/CXCR2 Paracrine Axis Contributes to Lung Metastasis in Osteosarcoma.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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