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NIT2抗体 [3D5]

KO/KD Overexpression
Anti-NIT2 antibody [3D5] used in IHC (Paraffin sections) (IHC-P). GTX84039
Anti-NIT2 antibody [3D5] used in Western Blot (WB). GTX84039
Anti-NIT2 antibody [3D5] used in IHC (Paraffin sections) (IHC-P). GTX84039
Anti-NIT2 antibody [3D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84039
Anti-NIT2 antibody [3D5] used in Flow cytometry (FACS). GTX84039
Anti-NIT2 antibody [3D5] used in Western Blot (WB). GTX84039
Anti-NIT2 antibody [3D5] used in Western Blot (WB). GTX84039
Anti-NIT2 antibody [3D5] used in IHC (Paraffin sections) (IHC-P). GTX84039

目录号 GTX84039

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 3D5

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-P, FACS

种属反应

 Human, Mouse, Rat, Monkey
产品包装
100 μl (¥7300)
查看所有 NIT2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

31 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.

纯化方式

Purified by affinity chromatography

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

nitrilase family member 2 , HEL-S-8a

细胞定位

Cytoplasm

功能与背景

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

数据库

研究领域

產品图片

Anti-NIT2 antibody [3D5] used in IHC (Paraffin sections) (IHC-P). GTX84039

GTX84039 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84039 NIT2 antibody [3D5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NIT2 antibody [3D5] used in Western Blot (WB). GTX84039

GTX84039 WB Image

Overexpression

WB analysis of HEK293T cells transfected with NIT2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84039 NIT2 antibody [3D5].
Loading : 5 ug per lane

Anti-NIT2 antibody [3D5] used in IHC (Paraffin sections) (IHC-P). GTX84039

GTX84039 IHC-P Image

IHC-P analysis of liver tissue using GTX84039 NIT2 antibody [3D5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NIT2 antibody [3D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84039

GTX84039 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with NIT2 plasmid using GTX84039 NIT2 antibody [3D5].

Anti-NIT2 antibody [3D5] used in Flow cytometry (FACS). GTX84039

GTX84039 FACS Image

FACS analysis of HEK293T cells transfected with either NIT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84039 NIT2 antibody [3D5].

Anti-NIT2 antibody [3D5] used in Western Blot (WB). GTX84039

GTX84039 WB Image

KO/KD

WB analysis of wild type and NIT2 knock out 293T cell lysate(10μg per lane) using GTX84039 NIT2 antibody [3D5].
Dilution : 1:500

Anti-NIT2 antibody [3D5] used in Western Blot (WB). GTX84039

GTX84039 WB Image

WB analysis of various cell lines using GTX84039 NIT2 antibody [3D5].
Loading : 35 ug per lane
Dilution : 1:200

Anti-NIT2 antibody [3D5] used in IHC (Paraffin sections) (IHC-P). GTX84039

GTX84039 IHC-P Image

IHC-P analysis of kidney tissue using GTX84039 NIT2 antibody [3D5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 7300
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