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NMDAR1抗体

Anti-NMDAR1 antibody used in Western Blot (WB). GTX133097
Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097
Anti-NMDAR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133097
Anti-NMDAR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133097
Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097

目录号 GTX133097

文献引用 5

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Mouse, Rat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

105 kDa. ( Note )

预测种属反应

Human, Chicken, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.37 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Mouse

免疫原

Synthetic peptide encompassing a sequence within the Extracellular domain of mouse NMDAR1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886824

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

glutamate receptor, ionotropic, NMDA1 (zeta 1) , GluN1 , GluRdelta1 , GluRzeta1 , M100174 , NMD-R1 , NMDAR1 , NR1 , Nmdar , Rgsc174

细胞定位

Cell membrane; Multi-pass membrane protein , Cell junction , synapse , postsynaptic cell membrane , postsynaptic cell membrane , postsynaptic density

功能与背景

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Modulated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

数据库

研究领域

產品图片

Anti-NMDAR1 antibody used in Western Blot (WB). GTX133097

GTX133097 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NMDAR1 antibody (GTX133097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097

GTX133097 ICC/IF Image

NMDAR1 antibody detects NMDAR1 protein at synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR1 protein stained by NMDAR1 antibody (GTX133097) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue:Fluoroshield with DAPI (GTX30920).
Anti-NMDAR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133097

GTX133097 IHC-P Image

NMDAR1 antibody detects NMDAR1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NMDAR1 antibody (GTX133097) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NMDAR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133097

GTX133097 IHC-Fr Image

NMDAR1 antibody detects NMDAR1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: NMDAR1 protein stained by NMDAR1 antibody (GTX133097) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NMDAR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133097

GTX133097 ICC/IF Image

NMDAR1 antibody detects NMDAR1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMDAR1 stained by NMDAR1 antibody (GTX133097) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

文献引用

Application Reference

Wiatrak B et al. Mol Biol Rep 2022; 49 (11) : 10443-10455 Impact of NMDA receptor activation on DNA damage in PC12 neuron-like cell cultures in the presence of β-amyloid peptides.
Ko CY et al. Front Aging Neurosci 2021; 13 : 774477 Alleviative Effect of Alpha-Lipoic Acid on Cognitive Impairment in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Rats.
实验应用 : WB
种属反应 : Rat
Lo Cascio F et al. J Biol Chem 2020; 295 (44) : 14807-14825 Modulating disease-relevant tau oligomeric strains by small molecules.
实验应用 : WB
种属反应 : Mouse
Algamal M et al. J Neurotrauma 2019; (Epub) Impact of Repetitive Mild Traumatic Brain Injury on Behavioral and Hippocampal Deficits in a Mouse Model of Chronic Stress.
实验应用 : WB
种属反应 : Mouse
Meng G et al. EBioMedicine 2019; Gut microbe-derived metabolite trimethylamine N-oxide activates the cardiac autonomic nervous system and facilitates ischemia-induced ventricular arrhythmia via two different pathways.
实验应用 : IHC-P
种属反应 : Dog
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PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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