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NOTCH1抗体 [4C9]

KO/KD Overexpression
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in Western Blot (WB). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020
Anti-NOTCH1 antibody [4C9] used in Western Blot (WB). GTX84020

目录号 GTX84020

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 4C9

同种型

 IgG2b

实验应用

 WB, IHC-P

种属反应

 Human
产品包装
100 μl (¥7300)
查看所有 NOTCH1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000
IHC-P 1:50-1:150
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

273 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1

纯化方式

Purified by affinity chromatography
From tissue culture supernatant

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1

细胞定位

Cell membrane

功能与背景

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.

数据库

研究领域

產品图片

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX84020 NOTCH1 antibody [4C9].

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of kidney tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of liver tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of breast tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of human bladder tissue using GTX84020 NOTCH1 antibody [4C9].

Anti-NOTCH1 antibody [4C9] used in Western Blot (WB). GTX84020

GTX84020 WB Image

Overexpression

WB analysis of HEK293T cells transfected with NOTCH1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84020 NOTCH1 antibody [4C9].
Loading : 5 ug per lane

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX84020 NOTCH1 antibody [4C9].

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of lung carcinoma tissue using GTX84020 NOTCH1 antibody [4C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NOTCH1 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX84020

GTX84020 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84020 NOTCH1 antibody [4C9].

Anti-NOTCH1 antibody [4C9] used in Western Blot (WB). GTX84020

GTX84020 WB Image

KO/KD

WB analysis of wild type and NOTCH1 knock out HeLa cell lysate(10μg per lane) using GTX84020 NOTCH1 antibody [4C9].
Dilution : 1:500

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 7300
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