产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
Observed MW
120 (NTM) , 300 (Full-langth) kDa.
产品说明
This antibody was raised against human NOTCH1 Intracellular domain.
预测种属反应
Rat(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Intracellular domain of human NOTCH1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2910046
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1
细胞定位
Cell membrane
功能与背景
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]
数据库
研究领域
產品图片
![Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683](/templates/default/js/lightbox/images/loading.gif "Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683")
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GTX636683 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NOTCH1 antibody [HL1281] (GTX636683) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636683 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NOTCH1 antibody [HL1281] (GTX636683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683](/upload/website/prouct_img/small/GTX636683/GTX636683_T-44536_20220114_WB_competitor_watermark_w_23061202_350.webp "Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683")
![Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683](/upload/website/prouct_img/small/GTX636683/GTX636683_44606_20220304_WB_w_23061202_766.webp "Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683")
![Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683](/upload/website/prouct_img/normal/GTX636683/GTX636683_T-44536_20220114_WB_competitor_watermark_w_23061202_350.webp "Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683")
![Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683](/upload/website/prouct_img/normal/GTX636683/GTX636683_44606_20220304_WB_w_23061202_766.webp "Anti-NOTCH1 antibody [HL1281] used in Western Blot (WB). GTX636683")
