产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human NPEPPS. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
aminopeptidase puromycin sensitive , AAP-S , MP100 , PSA
细胞定位
Cytoplasm, cytosol,Nucleus
功能与背景
This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005](/templates/default/js/lightbox/images/loading.gif "Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005")
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GTX637005 IHC-P Image
NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637005 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637005 WB Image
Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637005 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX637005 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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|
GTX637005 IHC-P Image
NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637005 ICC/IF Image
NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
The observed subcellular location is based on the publication: PMID: 28259985
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文献引用
目前没有使用 NPEPPS antibody [HL1531] (GTX637005) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
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![Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005](/upload/website/prouct_img/small/GTX637005/GTX637005_T-44690_20220617_IHC-P_M_22062121_591.webp "Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005](/upload/website/prouct_img/small/GTX637005/GTX637005_44753_20221209_WB_D_C_22121123_946.webp "Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005](/upload/website/prouct_img/small/GTX637005/GTX637005_44753_20221216_WB_Z_22122018_765.webp "Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005](/upload/website/prouct_img/small/GTX637005/GTX637005_T-44690_20220624_WB_M_R_22062919_101.webp "Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005](/upload/website/prouct_img/small/GTX637005/GTX637005_44753_20220729_WB_22080119_658.webp "Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005](/upload/website/prouct_img/small/GTX637005/GTX637005_44753_20220819_IHC-P_M_22090701_643.webp "Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637005](/upload/website/prouct_img/small/GTX637005/GTX637005_44753_20221021_ICC_IF_22110201_861.webp "Anti-NPEPPS antibody [HL1531] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005](/upload/website/prouct_img/normal/GTX637005/GTX637005_T-44690_20220617_IHC-P_M_22062121_591.webp "Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005](/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20221209_WB_D_C_22121123_946.webp "Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005](/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20221216_WB_Z_22122018_765.webp "Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005](/upload/website/prouct_img/normal/GTX637005/GTX637005_T-44690_20220624_WB_M_R_22062919_101.webp "Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005](/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20220729_WB_22080119_658.webp "Anti-NPEPPS antibody [HL1531] used in Western Blot (WB). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005](/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20220819_IHC-P_M_22090701_643.webp "Anti-NPEPPS antibody [HL1531] used in IHC (Paraffin sections) (IHC-P). GTX637005")
![Anti-NPEPPS antibody [HL1531] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637005](/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20221021_ICC_IF_22110201_861.webp "Anti-NPEPPS antibody [HL1531] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637005")
