产品说明
摘要
NRF2 antibody recognizes NRF2 protein, a basic leucine zipper region (bZip) transcription factor (predicted molecular weight of 68 kDa) that regulates expression of proteins with antioxidant and detoxification functions. NRF2 antibodies are commonly used to study oxidative stress, cell damage, metabolism, and immune response mechanisms, among others.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
1:100-1:500 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
Observed MW
110 kDa. The observed M.W. is based on the following publication. PMID: 22703241
阳性对照
3xFlag-human NFE2L2-transfected 293T , MDA-MB-231 nuclear extract , MDA-MB-231 nuclear extract (30uM tBHQ 4hr) , RAW264.7 (500 ng/ml LPS treatment for 6 hr)
预测种属反应
Bovine, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.65 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1950993
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
nuclear factor, erythroid 2 like 2 , HEBP1 , IMDDHH , NRF2
细胞定位
Cytoplasm , cytosol , Nucleus
功能与背景
NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]
数据库
研究领域
產品图片
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GTX103322 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX103322 WB Image
Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.
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GTX103322 ICC/IF Image
NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX103322 WB Image
NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103322 IHC-P Image
NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103322 ChIP assay Image
ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.
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GTX103322 WB Image
Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX103322 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103322 ICC/IF Image
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.
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GTX103322 ICC/IF Image
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX103322 ICC/IF Image
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.
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GTX103322 WB Image
Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103322 IP Image
Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal (GTX103322). Western blot analysis was performed using NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX103322 WB Image
Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX103322 IHC-P Image
The data was published in the journal Redox Biol in 2017.PMID: 28448946
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GTX103322 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27023634
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GTX103322 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27023634
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GTX103322 WB Image
The data was published in the journal PLoS One in 2013. PMID: 24204994
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GTX103322 WB Image
The data was published in the journal Cells in 2019. PMID: 31366086
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GTX103322 WB Image
The data was published in the journal Int J Mol Sci in 2017. PMID: 28926934
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文献引用
产品评论
NRF2 antibody [N2C2], Internal
目录号 GTX103322
Rating
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( Average 5 based on 2 users reviews)
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实验应用
Western Blot(WB)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 15-Nov-2022
Application Tested :
WB
Sample Species :
Ms
Sample :
mouse hepatocyte
Amount used :
50μg
Blocking :
5% nonfat milk, 20°C, 1Hr
Primary Antibody :
1:1000, 4°C, 12Hr
Date :
Anonymous submitted on 19-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
LNCaP cells
Amount used :
30 μg
Blocking :
5% non-fat milk in TBST, RT°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
条带说明 :
Lane1: Control (24 h after irradiation with 0 Gy)
Lane2: 24 h after irradiation with 2 Gy
Lane3: 24 h after irradiation with 4 Gy
Lane4: 24 h after irradiation with 8 Gy