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NSE抗体 [C1C3]

Anti-NSE antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113428
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428
Anti-NSE antibody [C1C3] used in IHC (Frozen sections) (IHC-Fr). GTX113428
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428
Anti-NSE antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113428
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428
Anti-NSE antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113428

目录号 GTX113428

文献引用 2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 NSE 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

47 kDa. ( Note )

阳性对照

Mouse brain , 293T , A431

产品说明

This antibody was raised against human NSE (ENO2) protein, but it may cross react other family members based on sequence homology.

预测种属反应

Bovine, Chicken, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human NSE. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950201

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

enolase 2 , HEL-S-279 , NSE

细胞定位

Cytoplasm , Cell membrane

功能与背景

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-NSE antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113428

GTX113428 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NSE antibody [C1C3] (GTX113428) at 1:300 dilution.

Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428

GTX113428 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
B: zebrafish muscle
10% SDS PAGE
GTX113428 diluted at 1:1000

Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428

GTX113428 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
10% SDS PAGE
GTX113428 diluted at 1:1000

Anti-NSE antibody [C1C3] used in IHC (Frozen sections) (IHC-Fr). GTX113428

GTX113428 IHC-Fr Image

NSE antibody [C1C3] detects NSE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NSE protein stained by NSE antibody [C1C3] (GTX113428) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428

GTX113428 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX113428 diluted at 1:10000

Anti-NSE antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113428

GTX113428 ICC/IF Image

NSE antibody [C1C3] detects NSE protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NSE protein stained by NSE antibody [C1C3] (GTX113428) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-NSE antibody [C1C3] used in Western Blot (WB). GTX113428

GTX113428 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [C1C3] (GTX113428) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSE antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113428

GTX113428 ICC/IF Image

The data was published in the journal Stem Cells Int in 2019. PMID: 31065279

文献引用

Application Reference

Cort?s-Medina LV et al. Stem Cells Int 2019; 2019 : 7627148 Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail.
Lorena V. Cort?s-Medina et al. Stem Cells International 2019; 2019 : Article ID 7627148 Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail.
实验应用 : ICC/IF
种属反应 : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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