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NSE抗体 [N1C1]

Orthogonal
Anti-NSE antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX101553
Anti-NSE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

目录号 GTX101553

文献引用 2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 NSE 的产品
产品说明

摘要

NSE is a 78 kDa gamma-homodimer expressed as a dominant enolase-isoenzyme in neuronal and neuroendocrine tissues. Elevated levels of serum NSE are often associated with neuronal disorders and neural crest-derived tumors. Up to 70 percent of patients with small cell lung carcinoma (SCLC) have high levels of serum NSE, as SCLC cells can express neuronal gene programs.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

47 kDa. ( Note )

阳性对照

Mouse brain

产品说明

This antibody was raised against human NSE (ENO2) protein, but it may cross react other family members based on sequence homology.

预测种属反应

Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human NSE. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240765

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

enolase 2 , HEL-S-279 , NSE

细胞定位

Cytoplasm , Cell membrane

功能与背景

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-NSE antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX101553

GTX101553 IHC-Fr Image

NSE antibody [N1C1] detects NSE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NSE protein stained by NSE antibody [N1C1] (GTX101553) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NSE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101553

GTX101553 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ENO2(GTX101553) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX101553 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 27034167

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

The data was published in the journal Oncotarget in 2016.PMID: 27034167

文献引用

Application Reference

Ugun-Klusek A et al. Cell Death Dis 2017; 8 (1) : e2531 Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway.
实验应用 : WB
种属反应 : Mouse
Lin TP et al. Oncotarget 2016; (Epub) REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling.
实验应用 : WB
种属反应 : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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