产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
For WB application, it works on S. cerevisiae and S. pombe nut may be not suitbale for human, T. etrahymena and yeasts. Recommend GTX00693 NUP98 antibody [13C2] on human, T. etrahymena and yeasts samples.
产品属性
形式
Liquid
存储溶液
Filter-sterilized PBS, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Tetrahymena
免疫原
Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila.
Peptide 1; 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29
Peptide 2; 646-SNPTQGGGLFGAANPGLGG-664
Epitope determined: GLF
纯化方式
Purified IgG
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
nucleoporin 98 , ADIR2 , NUP196 , NUP96
细胞定位
nucleoplasm
功能与背景
Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]
数据库
研究领域
產品图片
![Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695](/templates/default/js/lightbox/images/loading.gif "Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695")
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GTX00695 WB Image
WB analysis of S. cereviciae celll extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Dilution :
13C2 or 21A10 : 1:10
2H10 : 20 μg/ml
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GTX00695 WB Image
WB analysis of Tetrahymena themophila cells or Tetrahymena themophila cells overexpressing GFP-MacNup98A using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Diamonds and asterisks represent uncharacterized proteins.
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GTX00695 WB Image
WB analysis of HeLa whole cell lysate using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Dilution : 0.4 μg/ml
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GTX00695 Image
Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.
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GTX00695 ICC/IF Image
ICC/IF analysis of S. pombe cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Green : Primary antibody
Violet : DAPI
Fixation : 4% PFA for 10 min, treated with 0.6 mg/ml Zymolyase 100T at 3 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
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GTX00695 ICC/IF Image
ICC/IF analysis of Tetrahymena themophila cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. 13C2 mAb was highly specific to the macronucleus. In contrast, in addition to clear macronuclear staining, 21A10 mAb also stained the micronuclear periphery. This indicates that 21A10 mAb recognizes Nups localizing to the micronucleus such as Nup308 in addition to MacNup98A. Neither mABs 2H10 nor 414 could stain nuclear periphery of Tetrahymena.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
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GTX00695 WB Image
WB analysis of S. pombe cell l extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. Left and right lanes represent specimens from a wild type strain and an S. pombe strain in which Nup98 was chromosomally replaced with Nup98-GFP, respectively.
Dilution :
13C2 or 21A10 : 1:10
2H10 : 1 μg/ml
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GTX00695 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
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GTX00695 ICC/IF Image
ICC/IF analysis of S. cereviciae cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10].
Green : Primary antibody
Violet : DAPI
Dilution :
13C2 or 21A10 : 1:10
2H10 : 10 μg/ml
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文献引用
产品评论
目前没有使用 NUP98 antibody [21A10] (GTX00695) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695](/upload/website/prouct_img/small/GTX00695/GTX00695_20191104_WB_3_w_23053121_491.webp "Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695")
![Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695](/upload/website/prouct_img/small/GTX00695/GTX00695_20191104_WB_1_w_23053121_916.webp "Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695")
![Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695](/upload/website/prouct_img/small/GTX00695/GTX00695_20191104_WB_w_23053121_694.webp "Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695")
![Anti-NUP98 antibody [21A10] used in (). GTX00695](/upload/website/prouct_img/small/GTX00695/GTX00695_20191104_table_1_w_23053121_768.webp "Anti-NUP98 antibody [21A10] used in (). GTX00695")
![Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695](/upload/website/prouct_img/small/GTX00695/GTX00695_20191104_ICC-IF_2_w_23053121_493.webp "Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695")
![Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695](/upload/website/prouct_img/small/GTX00695/GTX00695_20191104_ICC-IF_1_w_23053121_871.webp "Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695")
![Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695](/upload/website/prouct_img/small/GTX00695/GTX00695_20191104_WB_2_w_23053121_268.webp "Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695")
![Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695](/upload/website/prouct_img/small/GTX00695/GTX00695_20191104_ICC-IF_w_23053121_358.webp "Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695")
![Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695](/upload/website/prouct_img/small/GTX00695/GTX00695_20191104_ICC-IF_3_w_23053121_421.webp "Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695")
![Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695](/upload/website/prouct_img/normal/GTX00695/GTX00695_20191104_WB_3_w_23053121_491.webp "Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695")
![Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695](/upload/website/prouct_img/normal/GTX00695/GTX00695_20191104_WB_1_w_23053121_916.webp "Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695")
![Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695](/upload/website/prouct_img/normal/GTX00695/GTX00695_20191104_WB_w_23053121_694.webp "Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695")
![Anti-NUP98 antibody [21A10] used in (). GTX00695](/upload/website/prouct_img/normal/GTX00695/GTX00695_20191104_table_1_w_23053121_768.webp "Anti-NUP98 antibody [21A10] used in (). GTX00695")
![Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695](/upload/website/prouct_img/normal/GTX00695/GTX00695_20191104_ICC-IF_2_w_23053121_493.webp "Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695")
![Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695](/upload/website/prouct_img/normal/GTX00695/GTX00695_20191104_ICC-IF_1_w_23053121_871.webp "Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695")
![Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695](/upload/website/prouct_img/normal/GTX00695/GTX00695_20191104_WB_2_w_23053121_268.webp "Anti-NUP98 antibody [21A10] used in Western Blot (WB). GTX00695")
![Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695](/upload/website/prouct_img/normal/GTX00695/GTX00695_20191104_ICC-IF_w_23053121_358.webp "Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695")
![Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695](/upload/website/prouct_img/normal/GTX00695/GTX00695_20191104_ICC-IF_3_w_23053121_421.webp "Anti-NUP98 antibody [21A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00695")
