ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min

WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10].
Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe.
Dilution : 2 μg/ml

ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min

Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.

ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10].
The dots are Nup98.

WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10].
Dilution : 1 μg/ml

ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10].
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10].
Dilution : 1:2000