产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Jurkat
预测种属反应
Mouse, Rat(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human NUP98. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10619089
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
nucleoporin 98 , ADIR2 , NUP196 , NUP96
细胞定位
Nucleus , nuclear pore complex , Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side
功能与背景
Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kD nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kD precursor protein. This cleavage results in the 98 kD nucleoporin as well as a 96 kD nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kD nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromsome translocatons in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-NUP98 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX114157](/templates/default/js/lightbox/images/loading.gif "Anti-NUP98 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX114157")
|
GTX114157 IP Image
Immunoprecipitation of NUP98 protein from HeLa whole cell extracts using 5 μg of NUP98 antibody [N3C1], Internal (GTX114157).
Western blot analysis was performed using NUP98 antibody [N3C1], Internal (GTX114157) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
|
|
|
GTX114157 WB Image
Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX114157 diluted at 1:1000
|
文献引用
目前没有使用 NUP98 antibody [N3C1], Internal (GTX114157) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 NUP98 antibody [N3C1], Internal (GTX114157) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-NUP98 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX114157](/upload/website/prouct_img/small/GTX114157/GTX114157_40247_IP_w_23060501_457.webp "Anti-NUP98 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX114157")
![Anti-NUP98 antibody [N3C1], Internal used in Western Blot (WB). GTX114157](/upload/website/prouct_img/small/GTX114157/GTX114157_WB_w_23060501_584.webp "Anti-NUP98 antibody [N3C1], Internal used in Western Blot (WB). GTX114157")
![Anti-NUP98 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX114157](/upload/website/prouct_img/normal/GTX114157/GTX114157_40247_IP_w_23060501_457.webp "Anti-NUP98 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX114157")
![Anti-NUP98 antibody [N3C1], Internal used in Western Blot (WB). GTX114157](/upload/website/prouct_img/normal/GTX114157/GTX114157_WB_w_23060501_584.webp "Anti-NUP98 antibody [N3C1], Internal used in Western Blot (WB). GTX114157")
