NANOG antibody [GT3312] validation by siRNA knock-down.
Upperpanel: NANOG antibody [GT3312] GTX627421
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg Tera-2 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells
C. 30 μg whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells
D. 30 μg whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
GAPDH antibody (GTX100118) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sample (20 μg of whole cell lysate)
A: mouse ESC
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50

NANOG antibody [GT3312] detects NANOG protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nanog antibody [GT3312] (GTX627421) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

The data was published in the journal Mol Cancer in 2013. PMID: 24094005

The data was published in the journal Cancers (Basel) in 2019. PMID: 31878324