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Nanog抗体 [GT3312]

KO/KD
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Flow cytometry (FACS). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

目录号 GTX627421

文献引用 26
2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT3312

同种型

 IgG2b

实验应用

 WB, ICC/IF, FACS

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Nanog 的产品
产品说明

摘要

Nanog antibody recognizes Nanog protein, a 35 kDa transcription factor that maintains pluripotency and the self-renewal feature of embryonic stem cells. It is one of the Thomson factors (i.e., NANOG, OCT4, SOX2, and LIN28) that induce a stable intrinsic pluripotency network to generate induced pluripotent stem cells. In addition, NANOG expression is elevated in cancer stem cells and correlates with a worse prognosis for many tumor types.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
FACS 1:50-1:200
以下为常规应用缩写的中文注解
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

35 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , human ESC , mouse ESC , Tera-2

预测种属反应

Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

保存剂

No preservative

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_11169335

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Nanog homeobox

细胞定位

Nucleus

功能与背景

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

数据库

研究领域

產品图片

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

KO/KD

NANOG antibody [GT3312] validation by siRNA knock-down.
Upperpanel: NANOG antibody [GT3312] GTX627421
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg Tera-2 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells
C. 30 μg whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells
D. 30 μg whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
GAPDH antibody (GTX100118) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nanog antibody [GT3312] (GTX627421) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

NANOG antibody [GT3312] detects NANOG protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nanog antibody [GT3312] used in Flow cytometry (FACS). GTX627421

GTX627421 FACS Image

NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

Sample (20 μg of whole cell lysate)
A: mouse ESC
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

The data was published in the journal Cancers (Basel) in 2019. PMID: 31878324

Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421

GTX627421 WB Image

The data was published in the journal Mol Cancer in 2013. PMID: 24094005

文献引用

Application Reference

CH Hung et al. Nat Commun 2024; 15 (1) : 7885 Defective N-glycosylation of IL6 induces metastasis and tyrosine kinase inhibitor resistance in lung cancer
Peng Ye et al. Biomolecules 2022; 12 (5) Alanine-Glyoxylate Aminotransferase Sustains Cancer Stemness Properties through the Upregulation of SOX2 and OCT4 in Hepatocellular Carcinoma Cells.
实验应用 : WB
种属反应 : Human
Hassan G et al. Sci Rep 2022; 12 (1) : 2711 The significance of ErbB2/3 in the conversion of induced pluripotent stem cells into cancer stem cells.
实验应用 : WB
种属反应 : Human
Candice Merle et al. BMC Cancer 2021; 21 (1) : 241 Acquisition of cancer stem cell capacities after spontaneous cell fusion.
实验应用 : WB
种属反应 : Human
Ciarpella F et al. iScience 2021; 24 (12) : 103438 Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.
种属反应 : Mouse
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产品评论

Nanog antibody [GT3312] 目录号 GTX627421

Rating
( Average 4.5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-Nanog antibody [GT3312] used in Western Blot (WB). GTX627421
Date : Anonymous submitted on 28-Jun-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : human iPSC cells
Amount used : 6μg
Anti-Nanog antibody [GT3312] used in Flow cytometry (FACS). GTX627421
Date : Anonymous submitted on 11-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Black: Isotype control, Green: NANOG antibody
我要评论产品
Application Reference
CH Hung et al. Nat Commun 2024; 15 (1):7885 Defective N-glycosylation of IL6 induces metastasis and tyrosine kinase inhibitor resistance in lung cancer
Peng Ye et al. Biomolecules 2022; 12 (5) Alanine-Glyoxylate Aminotransferase Sustains Cancer Stemness Properties through the Upregulation of SOX2 and OCT4 in Hepatocellular Carcinoma Cells.
实验应用 : WB
种属反应 : Human
Hassan G et al. Sci Rep 2022; 12 (1):2711 The significance of ErbB2/3 in the conversion of induced pluripotent stem cells into cancer stem cells.
实验应用 : WB
种属反应 : Human
Candice Merle et al. BMC Cancer 2021; 21 (1):241 Acquisition of cancer stem cell capacities after spontaneous cell fusion.
实验应用 : WB
种属反应 : Human
Ciarpella F et al. iScience 2021; 24 (12):103438 Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.
种属反应 : Mouse
Biagioni A et al. Front Oncol 2021; 11:663225 CRISPR/Cas9 uPAR Gene Knockout Results in Tumor Growth Inhibition, EGFR Downregulation and Induction of Stemness Markers in Melanoma and Colon Carcinoma Cell Lines.
实验应用 : WB
种属反应 : Human
Walker SJ et al. Heliyon 2021; 7 (4):e06617 A simplified approach for derivation of induced pluripotent stem cells from Epstein-Barr virus immortalized B-lymphoblastoid cell lines.
实验应用 : ICC/IF
种属反应 : Human
Chen YL et al. Sci Rep 2021; 11 (1):644 Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells.
实验应用 : WB
种属反应 : Human
Jao TM et al. Cancer Lett 2021; 499:290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.
实验应用 : WB
种属反应 : Human
Ram?rez-Renter?a C et al. Case Rep Endocrinol 2020; 2020:4768281 From ACTH-Dependent to ACTH-Independent Cushing's Syndrome from a Malignant Mixed Corticomedullary Adrenal Tumor: Potential Role of Embryonic Stem Cells.
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
实验应用 : WB
种属反应 : Human
Blanas A et al. Cancers (Basel) 2020; 12 (9) FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State.
实验应用 : FACS
种属反应 : Mouse
Andreucci E et al. J Mol Med (Berl) 2020; 98 (10):1431-1446 The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells.
实验应用 : FACS
种属反应 : Human
Chen YL et al. Cancers (Basel) 2019; 12 (1) ERK Activation Modulates Cancer Stemness and Motility of a Novel Mouse Oral Squamous Cell Carcinoma Cell Line.
Chen YH et al. Sci Rep 2019; 9 (1):9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.
实验应用 : ICC/IF
种属反应 : Human
Yang CE et al. Chem Biol Interact 2019; 302:28-35 the antipsychotic * suppresses yap signaling, stemness properties, and drug resistance in breast cancer cells.
实验应用 : WB
种属反应 : Human
Lin YH et al. Endocr Relat Cancer 2018; 25 (12):967-979 Thyroid hormone negatively regulates tumorigenesis through suppression of BC200.
实验应用 : WB
种属反应 : Human
Fan JR et al. Cell Death Dis 2018; 9 (5):502 Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
实验应用 : FACS
种属反应 : Human
Zhang Y.X. et al. Transplantation Proceedings 2018; (Epub) Development of Individualized Induced Pluripotent Stem Cells from Fibroblasts of Keloid Lesions in Patients’.
实验应用 : ICC/IF
种属反应 : Human
Chang KY et al. Biochem Biophys Res Commun 2017; 493 (1):14-19 Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.
实验应用 : WB
种属反应 : Human
Ko CY et al. Mol Neurobiol 2017; (Epub) MDM2 Degrades Deacetylated Nucleolin Through Ubiquitination to Promote Glioma Stem-Like Cell Enrichment for Chemotherapeutic Resistance.
实验应用 : WB
种属反应 : Human
Gupta N et al. Proc Natl Acad Sci U S A 2017; 114 (5):1033-1038 Stress granule-associated protein G3BP2 regulates breast tumor initiation.
实验应用 : WB
种属反应 : Human
Huang TT et al. Oncotarget 2015; 6 (20):18012-26 The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.
实验应用 : WB
种属反应 : Human
Wang AM et al. Oncotarget 2014; 5 (13):5002-16 Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.
实验应用 : WB
种属反应 : Human
Liu KC et al. Oncotarget 2013; 4 (12):2366-82 Ovarian cancer stem-like cells show induced translineage-differentiation capacity and are suppressed by alkaline phosphatase inhibitor.
实验应用 : FACS
种属反应 : Human
Huang AF et al. Mol Cancer 2013; 12 (1):115 CD164 regulates the tumorigenesis of ovarian surface epithelial cells through the SDF-1α/CXCR4 axis.
实验应用 : WB
种属反应 : Human
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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