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Nek1抗体

KO/KD Orthogonal
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Anti-Nek1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130828
Anti-Nek1 antibody used in Western Blot (WB). GTX130828

目录号 GTX130828

文献引用 1
3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IP

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

143 kDa. ( Note )

Observed MW

180 kDa. The observed M.W. is based on the following publication. PMID: 16603261

阳性对照

293T , mouse brain , rat brain

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Nek1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886357

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

NIMA related kinase 1 , ALS24 , NY-REN-55 , SRPS2 , SRPS2A , SRTD6

细胞定位

Nucleus , Cytoplasm

功能与背景

The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

数据库

研究领域

產品图片

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

KO/KD

Wild-type (WT) and NEK1 knockout (KO) HeLa cell extracts (100 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nek1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130828

GTX130828 ICC/IF Image

Nek1 antibody detects Nek1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nek1 protein stained by Nek1 antibody (GTX130828) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Nek1 antibody used in Western Blot (WB). GTX130828

GTX130828 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 16603261

文献引用

Application Reference

Freund I et al. Cells 2020; 9 (5) Fractionation-Dependent Radiosensitization by Molecular Targeting of Nek1.
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产品评论

Nek1 antibody 目录号 GTX130828

Rating
( Average 5 based on 3 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Nek1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130828
Date : Anonymous submitted on 09-Jun-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa WT and NEK1 KO cells
Blocking : 5% BSA, 5% goat serum and 0.01% Triton X-100 in PBS, 30min, RT
Fixation : 4% PFA in PBS, 15 min, RT
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 10 min, RT
Primary Antibody : 1:300, 4°C, > 12Hr
条带说明 :
Yellow dashed line: HeLa WT cells
Magenta dashed line: HeLa KO cells
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Nek1 antibody used in Immunoprecipitation (IP). GTX130828
Date : Anonymous submitted on 09-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HeLa cells
Primary Antibody : Undiluted, 4°C, > 12Hr
条带说明 :
SM: 10% starting material
UB: 10% unbound fraction
IP: IP antibody
Figure Note : Antibody-bead conjugates were prepared by adding 1.0 μg of antibody to 500 μl of PBS with 0.01% triton X-100, together with 30μl of Dynabeads Protein A-Sepharose beads. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Nek1 antibody used in Western Blot (WB). GTX130828
Date : Anonymous submitted on 09-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa WT and NEK1 KO cells
Amount used : 100μg
Primary Antibody : 1:1000, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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