Nestin抗体 [GT623]

目录号 GTX630201
目录号  GTX630201
Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
GTX630201 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
GTX630201 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201
GTX630201 ICC/IF Image

Nestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.
Green: Nestin stained by Nestin antibody [GT623] (GTX630201) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201
  • 宿主
    Mouse
  • 克隆
    Monoclonal
  • 克隆编号
    GT623
  • 同种型
    IgG1
  • 实验应用
    WB ICC/IF
  • 种属反应
    Human
摘要
Nestin antibody recognizes nestin, a type-IV intermediate filament protein that migrates at ~240 kDa on western blot. Nestin is an established marker of mesodermal and neuroectodermal fates, and is expressed in neural stem cells. It is also enriched in endothelial cells, regardless of their proliferative state.
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