产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human NeuN. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
RNA binding fox-1 homolog 3 , FOX-3 , FOX3 , HRNBP3 , NEUN
细胞定位
Nucleus,Cytoplasm
功能与背景
This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]
数据库
研究领域
產品图片
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GTX638198 IHC-P Image
NeuN antibody [HL2194] detects NeuN protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.NeuN stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638198 IHC-Fr Image
NeuN antibody [HL2194] detects NeuN protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Frozen-sectioned mouse spinal cord.Green: NeuN stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638198 ICC/IF Image
NeuN antibody [HL2194] detects NeuN protein at nucleus by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NeuN stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:500.Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638198 IHC-P Image
NeuN antibody [HL2194] detects NeuN protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.NeuN stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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