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Nuclear Matrix Protein p84抗体 [5E10]

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Immunoprecipitation (IP). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in IHC (Paraffin sections) (IHC-P). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in IHC (Paraffin sections) (IHC-P). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220
Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

目录号 GTX70220

宿主

Mouse

克隆

Monoclonal

克隆编号

5E10

同种型

IgG2b

实验应用

WB, ICC/IF, IHC-P, IP, ChIP assay, IHC

种属反应

Human, Mouse, Rat, Hamster, Monkey
产品包装
100 μl (¥4300)
产品说明

摘要

Nuclear Matrix Protein p84 antibody detects THO complex subunit 1, which is an 84 kDa nuclear matrix protein often referred to as just “p84”. p84 is involved in mRNA transcription and is considered to be a nuclear marker. p84 antibody is an effective reagent used to verify the purity of nuclear extracts in cellular fractionation assays.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 0.3 - 2 μg/ml
ICC/IF 0.5 - 2 μg/ml
IHC-P Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

76 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , A375 , HeLa nucleus , NIH-3T3 , mouse brain , PC-12 , mouse Cerebellum

产品说明

Knockdown/Knockout validation was supported by references (PMID:25296641)

产品属性

形式

Liquid

存储溶液

1XPBS pH7, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Amino acids 15-374 of human p84 expressed in E. coli.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_372637

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

THO complex 1 , HPR1 , P84 , P84N5

细胞定位

nucleoplasm , Cytoplasm

功能与背景

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).[supplied by OMIM, Nov 2010]

数据库

研究领域

產品图片

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Immunoprecipitation (IP). GTX70220

GTX70220 IP Image

p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] (GTX70220) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (GTX70220) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in IHC (Paraffin sections) (IHC-P). GTX70220

GTX70220 IHC-P Image

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70220

GTX70220 ICC/IF Image

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in IHC (Paraffin sections) (IHC-P). GTX70220

GTX70220 IHC-P Image

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody (GTX70220) in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Sample (50 μg of whole cell lysate)
A: mouse Cerebellum
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
B: HeLa nucleus
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal PLoS One in 2013.PMID: 23805200

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the Nat Commun in 2020. PMID: 33257668

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal BMC Biochem in 2007. PMID: 17880698

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal Nat Commun in 2016.PMID: 27046438

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal Nat Commun in 2016.PMID: 27046438

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the 2019 in Elife. PMID: 31663849

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the 2019 in Elife. PMID: 31663849

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal EMBO Mol Med in 2013. PMID: 23341130

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal J Biomed Sci in 2014. PMID: 25030234

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal EMBO Mol Med in 2013. PMID: 23341130

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal Biomed Res Int in 2015. PMID: 25834829

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28157211

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28157211

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29045486

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29045486

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal Biomed Res Int in 2015. PMID: 25722974

Anti-Nuclear Matrix Protein p84 antibody [5E10] used in Western Blot (WB). GTX70220

GTX70220 WB Image

The data was published in the journal PLoS Pathog in 2015. PMID: 25927232

文献引用

产品评论

Nuclear Matrix Protein p84 antibody [5E10] 目录号 GTX70220

Rating ( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Feb-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 30μg
Blocking : TBST with 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Application Reference
Min Chen et al. bioRxiv 2019; (Epub)
实验应用 : WB
种属反应 : Human
Clone Reference
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4300
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