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Nucleobindin 1抗体 [HL2532]

Recombinant antibody KO/KD Orthogonal
Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in IHC (Paraffin sections) (IHC-P). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in IHC (Paraffin sections) (IHC-P). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901
Anti-Nucleobindin 1 antibody [HL2532] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638901

目录号 GTX638901

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2532

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

54 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment of human Nucleobindin 1

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

nucleobindin 1 , CALNUC , NUC

细胞定位

Cytoplasm,Secreted

功能与背景

This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]

数据库

研究领域

產品图片

Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901

GTX638901 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901

GTX638901 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Nucleobindin 1 antibody [HL2532] used in IHC (Paraffin sections) (IHC-P). GTX638901

GTX638901 IHC-P Image

Nucleobindin 1 antibody [HL2532] detects secreted Nucleobindin 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nucleobindin 1 antibody [HL2532] used in IHC (Paraffin sections) (IHC-P). GTX638901

GTX638901 IHC-P Image

Nucleobindin 1 antibody [HL2532] detects secreted Nucleobindin 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Nucleobindin 1 antibody [HL2532] used in Western Blot (WB). GTX638901

GTX638901 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Nucleobindin 1 antibody [HL2532] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638901

GTX638901 ICC/IF Image

Nucleobindin 1 antibody [HL2532] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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