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O-GlcNAc transferase抗体 [HL1641]

Recombinant antibody KO/KD Orthogonal
Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in IHC (Paraffin sections) (IHC-P). GTX637222
Anti-O-GlcNAc transferase antibody [HL1641] used in IHC (Paraffin sections) (IHC-P). GTX637222

目录号 GTX637222

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1641

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat, Drosophila
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

117 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

O-linked N-acetylglucosamine (GlcNAc) transferase , HINCUT-1 , HRNT1 , MRX106 , O-GLCNAC , OGT1

细胞定位

Nucleus,Cytoplasm,Membrane,Cell membrane

功能与背景

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

数据库

研究领域

產品图片

Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222

GTX637222 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222

GTX637222 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222

GTX637222 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-O-GlcNAc transferase antibody [HL1641] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637222

GTX637222 ICC/IF Image

O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-O-GlcNAc transferase antibody [HL1641] used in Western Blot (WB). GTX637222

GTX637222 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-O-GlcNAc transferase antibody [HL1641] used in IHC (Paraffin sections) (IHC-P). GTX637222

GTX637222 IHC-P Image

O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-O-GlcNAc transferase antibody [HL1641] used in IHC (Paraffin sections) (IHC-P). GTX637222

GTX637222 IHC-P Image

O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (GTX637222) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Su WC et al. In Vivo 2023; 37 (6) : 2524-2532 Thiamet G as a Potential Treatment for Polycystic Kidney Disease.
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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