产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse brain , 293T , A431 , HeLa , HepG2
产品说明
Based on sequence homology, this antibody may cross react with OGDHL protein.
预测种属反应
Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human OGDH. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1951054
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
oxoglutarate dehydrogenase , AKGDH , E1k , OGDC
细胞定位
Mitochondrion matrix
功能与背景
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX105124 WB Image
Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX105124 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105124 WB Image
OGDH antibody detects OGDH protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and blotted with OGDH antibody (GTX105124) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105124 IHC-P Image
OGDH antibody [C2C3], C-term detects OGDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.OGDH stained by OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105124 ICC/IF Image
OGDH antibody [C2C3], C-term detects OGDH protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: OGDH stained by OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:200.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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GTX105124 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105124 IHC-P Image
OGDH antibody [C2C3], C-term detects OGDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.OGDH stained by OGDH antibody [C2C3], C-term (GTX105124) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105124 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using OGDH(GTX105124) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX105124 IHC-P Image
OGDH antibody [C2C3], C-term detects OGDH protein at mitochondria on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. OGDH antibody [C2C3], C-term (GTX105124) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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