产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human OGDH protein, and it does not cross react with human OGDHL protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human OGDH.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
oxoglutarate dehydrogenase , AKGDH , E1k , OGDC
细胞定位
Mitochondrion matrix,Nucleus
功能与背景
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
数据库
研究领域
產品图片
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/templates/default/js/lightbox/images/loading.gif "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
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GTX637980 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [HL2072] (GTX637980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637980 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [HL2072] (GTX637980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637980 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [HL2072] (GTX637980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637980 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [HL2072] (GTX637980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637980 ICC/IF Image
OGDH antibody [HL2072] detects OGDH protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: OGDH stained by OGDH antibody [HL2072] (GTX637980) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20240202_WB_multiple_B_24021917_358.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20240223_WB_TPM_watermark_24022619_584.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20240223_WB_M_tissue_24022619_323.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20240223_WB_R_muscle_24022619_216.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20230317_ICC_IF_24030600_347.webp "Anti-OGDH antibody [HL2072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20240202_WB_multiple_B_24021917_358.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20240223_WB_TPM_watermark_24022619_584.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20240223_WB_M_tissue_24022619_323.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20240223_WB_R_muscle_24022619_216.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20230317_ICC_IF_24030600_347.webp "Anti-OGDH antibody [HL2072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637980")
