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OLIG2抗体

Anti-OLIG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132732
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

目录号 GTX132732

文献引用 6
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat, Pig
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

32 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human OLIG2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886726

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

oligodendrocyte transcription factor 2 , BHLHB1 , OLIGO2 , PRKCBP2 , RACK17 , bHLHe19

细胞定位

Nucleus , Cytoplasm

功能与背景

This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-OLIG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132732

GTX132732 ICC/IF Image

OLIG2 antibody detects OLIG2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: OLIG2 protein stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

GTX132732 IHC-P Image

OLIG2 antibody detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
OLIG2 stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

GTX132732 IHC-P Image

OLIG2 antibody detects OLIG2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
OLIG2 stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

GTX132732 IHC-P Image

OLIG2 antibody detects OLIG2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: OLIG2 stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-OLIG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX132732

GTX132732 IHC-P Image

OLIG2 antibody detects OLIG2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: OLIG2 stained by OLIG2 antibody (GTX132732) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Kakae M et al. Sci Adv 2023; 9 (29) : eadh0102 The astrocytic TRPA1 channel mediates an intrinsic protective response to vascular cognitive impairment via LIF production.
Chand K.K. et al. bioRxiv 2021; (Epub) Human primed endothelial colony forming cells exert neuroprotective effects in the growth restricted newborn piglet.
实验应用 : IHC-P
种属反应 : Pig
Lee JK et al. J Neuropathol Exp Neurol 2021; 80 (2) : 182-198 Combining Hypothermia and Oleuropein Subacutely Protects Subcortical White Matter in a Swine Model of Neonatal Hypoxic-Ischemic Encephalopathy.
实验应用 : WB
种属反应 : Pig
Barati S et al. Neuropeptides 2019; 75 : 75-84 Effect of mesenchymal stem cells on glial cells population in cuprizone induced demyelination model.
实验应用 : IHC-P
种属反应 : Mouse
Baker EW et al. J Neurotrauma 2018; (Epub) Controlled Cortical Impact Severity Results in Graded Cellular, Tissue, and Functional Responses in a Piglet Traumatic Brain Injury Model.
实验应用 : IHC-P
种属反应 : Pig
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产品评论

OLIG2 antibody 目录号 GTX132732

Rating
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实验应用
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-OLIG2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132732
Date : Tanusree Sen submitted on 26-Dec-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Brain tissue
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, 25°C, 0.5Hr
Primary Antibody : 1:1:100, 4°C, 12Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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