GeneTex中国官方网站
  • China

Oct4抗体 [GT486]

KO/KD Orthogonal Overexpression
Anti-Oct4 antibody [GT486] used in Immunoprecipitation (IP). GTX627419
Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Flow cytometry (FACS). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419
Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

目录号 GTX627419

文献引用 16
2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT486

同种型

 IgG2a

实验应用

 WB, ICC/IF, IHC-P, FACS, IP

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Oct4 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:1000-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

39 kDa. ( Note )

阳性对照

LGNSO transfected HeLa , human ESC

预测种属反应

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.66 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.
From tissue culture supernatant

偶联

Unconjugated

RRID

AB_11176459

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

细胞定位

Nucleus

功能与背景

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Oct4 antibody [GT486] used in Immunoprecipitation (IP). GTX627419

GTX627419 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627419) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627419) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

GTX627419 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4(GTX627419) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Mouse embryonic stem cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

GTX627419 IHC-P Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

GTX627419 IHC-P Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
(GTX627419) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Flow cytometry (FACS). GTX627419

GTX627419 FACS Image

Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Overexpression

Sample (10 μg of whole cell lysate)
A: Non-transfected HeLa lysates
B: HeLa transfected recombinant Oct4 protein
10% SDS PAGE
GTX627419 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419

GTX627419 ICC/IF Image

Oct4 antibody detects Oct4 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX627419) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419

GTX627419 ICC/IF Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

Orthogonal

The data was published in the 2020 in Sci Rep. PMID: 32398775

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

GTX627419 WB Image

The data was published in the journal Cancers (Basel) in 2019. PMID: 31878324

文献引用

Application Reference

Masola V et al. Front Oncol 2022; 12 : 918419 Heparanase regulates EMT and cancer stem cell properties in prostate tumors.
实验应用 : WB
种属反应 : Human
Mashiko T et al. Cells 2021; 10 (3) Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
实验应用 : ICC/IF
种属反应 : Human
Ram?rez-Renter?a C et al. Case Rep Endocrinol 2020; 2020 : 4768281 From ACTH-Dependent to ACTH-Independent Cushing's Syndrome from a Malignant Mixed Corticomedullary Adrenal Tumor: Potential Role of Embryonic Stem Cells.
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
实验应用 : WB
种属反应 : Human
Andreucci E et al. J Mol Med (Berl) 2020; 98 (10) : 1431-1446 The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells.
实验应用 : FACS
种属反应 : Human
显示更多 +
我要回报文献

产品评论

Oct4 antibody [GT486] 目录号 GTX627419

Rating
( Average 4 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 60μg
Blocking : 5% milk, 24°C, 1.5Hr
Primary Antibody : 1:1200, 4°C, 16Hr
Anti-Oct4 antibody [GT486] used in Flow cytometry (FACS). GTX627419
Date : Anonymous submitted on 16-May-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
我要评论产品
Application Reference
Masola V et al. Front Oncol 2022; 12:918419 Heparanase regulates EMT and cancer stem cell properties in prostate tumors.
实验应用 : WB
种属反应 : Human
Mashiko T et al. Cells 2021; 10 (3) Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
实验应用 : ICC/IF
种属反应 : Human
Ram?rez-Renter?a C et al. Case Rep Endocrinol 2020; 2020:4768281 From ACTH-Dependent to ACTH-Independent Cushing's Syndrome from a Malignant Mixed Corticomedullary Adrenal Tumor: Potential Role of Embryonic Stem Cells.
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
实验应用 : WB
种属反应 : Human
Andreucci E et al. J Mol Med (Berl) 2020; 98 (10):1431-1446 The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells.
实验应用 : FACS
种属反应 : Human
Aminuddin A et al. Sci Rep 2020; 10 (1):7885
实验应用 : WB
种属反应 : Human
Wang X et al. Stem Cell Res 2020; 42:101703 Generation of an iPSC line (HUSTi002-A) from fibroblasts of a patient with Sertoli cell-only syndrome carrying c.731_732delAT in PIWIL2 gene.
实验应用 : ICC/IF
种属反应 : Human
Chen YL et al. Cancers (Basel) 2019; 12 (1) ERK Activation Modulates Cancer Stemness and Motility of a Novel Mouse Oral Squamous Cell Carcinoma Cell Line.
Chien CH et al. J Biomed Sci 2019; 26 (1):77 Enrichment of superoxide dismutase 2 in glioblastoma confers to acquisition of temozolomide resistance that is associated with tumor-initiating cell subsets.
实验应用 : WB
种属反应 : Human
Huang TH et al. J Cell Mol Med 2019; 23 (12):8184-8195 In silico identification of thiostrepton as an inhibitor of cancer stem cell growth and an enhancer for chemotherapy in non-small-cell lung cancer.
实验应用 : WB
种属反应 : Human
Chen YH et al. Sci Rep 2019; 9 (1):9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.
实验应用 : ICC/IF
种属反应 : Human
Yang CE et al. Chem Biol Interact 2019; 302:28-35 the antipsychotic * suppresses yap signaling, stemness properties, and drug resistance in breast cancer cells.
实验应用 : WB
种属反应 : Human
Gupta N et al. Proc Natl Acad Sci U S A 2017; 114 (5):1033-1038 Stress granule-associated protein G3BP2 regulates breast tumor initiation.
实验应用 : WB
种属反应 : Human
Huang TT et al. Oncotarget 2015; 6 (20):18012-26 The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.
实验应用 : WB
种属反应 : Human
Wang AM et al. Oncotarget 2014; 5 (13):5002-16 Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.
实验应用 : WB
种属反应 : Human
Qin H et al. Cell 2014; 158 (2):449-61 Systematic Identification of Barriers to Human iPSC Generation.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com