Oct4抗体 [GT486]

Name: Oct4抗体 [GT486]
Brand: GeneTex
SKU: GTX627419
Price: 4000.00 RMB
Rating: 4.3 (3 reviews)

Anti-Oct4 antibody [GT486] used in Immunoprecipitation (IP). GTX627419

Anti-Oct4 antibody [GT486] used in Western Blot (WB). GTX627419

Anti-Oct4 antibody [GT486] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627419

Anti-Oct4 antibody [GT486] used in IHC (Paraffin sections) (IHC-P). GTX627419

Anti-Oct4 antibody [GT486] used in Flow cytometry (FACS). GTX627419

目录号 GTX627419

文献引用 16

宿主	Mouse
克隆	Monoclonal
克隆编号	GT486
同种型	IgG2a
实验应用	WB, ICC/IF, IHC-P, FACS, IP
种属反应	Human, Mouse
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 Oct4 的产品

View product citations for antibody GTX627419 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
FACS	1:50-1:200
IP	1:1000-1:1000

以下为常规应用缩写的中文注解
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

Observed MW

45 kDa.

阳性对照

LGNSO transfected HeLa , human ESC

预测种属反应

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

保存剂

No preservative

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.
From tissue culture supernatant

偶联

Unconjugated

RRID

AB_11176459

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

细胞定位

Nucleus

功能与背景

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

数据库

Gene ID: 5460 POU5F1
UniProt: Q01860 POU5F1

研究领域

產品图片

GTX627419 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627419) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627419) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX627419 WB Image

Sample (10 μg of whole cell lysate)
A: Non-transfected HeLa lysates
B: HeLa transfected recombinant Oct4 protein
10% SDS PAGE
GTX627419 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627419 ICC/IF Image

Oct4 antibody detects Oct4 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX627419) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX627419 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4(GTX627419) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX627419 IHC-P Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX627419 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627419 IHC-P Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX627419 WB Image

Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
(GTX627419) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627419 FACS Image

Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50

GTX627419 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627419 ICC/IF Image

Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.

GTX627419 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627419 WB Image

The data was published in the 2020 in Sci Rep. PMID: 32398775

GTX627419 WB Image

The data was published in the journal Cancers (Basel) in 2019. PMID: 31878324

文献引用

Application Reference

Masola V et al. Front Oncol 2022; 12 : 918419 Heparanase regulates EMT and cancer stem cell properties in prostate tumors.

实验应用 : WB

种属反应 : Human

Mashiko T et al. Cells 2021; 10 (3) Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.

实验应用 : ICC/IF

种属反应 : Human

Ram?rez-Renter?a C et al. Case Rep Endocrinol 2020; 2020 : 4768281 From ACTH-Dependent to ACTH-Independent Cushing's Syndrome from a Malignant Mixed Corticomedullary Adrenal Tumor: Potential Role of Embryonic Stem Cells.

Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.

实验应用 : WB

种属反应 : Human

Andreucci E et al. J Mol Med (Berl) 2020; 98 (10) : 1431-1446 The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells.

实验应用 : FACS

种属反应 : Human

显示更多 +

我要回报文献

产品评论

Oct4 antibody [GT486] 目录号 GTX627419

Rating

( Average 4.3 based on 3 users reviews)

实验应用

Western Blot(WB)		( Average 4.5 based on 2 users reviews)
Flow cytometry(FACS)		( Average 4 based on 1 users reviews)

Date : Anonymous submitted on 30-May-2024

Score :

Application Tested : WB

Sample Species : Hu

Sample : human iPSC cells

Amount used : 6μg

Blocking : 5% milk, 25°C, 1Hr

Primary Antibody : 1:1000, 4°C, 16Hr

Date : Anonymous submitted on 17-Jun-2016

Score :

Application Tested : WB

Sample Species : Hu

Sample : HeLa cells

Amount used : 60μg

Blocking : 5% milk, 24°C, 1.5Hr

Primary Antibody : 1:1200, 4°C, 16Hr

Date : Anonymous submitted on 16-May-2013

Score :

Application Tested : FACS

Sample Species : Hu

Sample : hES cells

Blocking : 2% serum, 4°C, 0.5Hr

Fixation : 70% ethanol

Primary Antibody : 1:50, 4°C, 0.5Hr

我要评论产品

Application Reference

Masola V et al. Front Oncol 2022; 12:918419 Heparanase regulates EMT and cancer stem cell properties in prostate tumors.

实验应用 : WB

种属反应 : Human

Mashiko T et al. Cells 2021; 10 (3) Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.

实验应用 : ICC/IF

种属反应 : Human

Ram?rez-Renter?a C et al. Case Rep Endocrinol 2020; 2020:4768281 From ACTH-Dependent to ACTH-Independent Cushing's Syndrome from a Malignant Mixed Corticomedullary Adrenal Tumor: Potential Role of Embryonic Stem Cells.

Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.

实验应用 : WB

种属反应 : Human

Andreucci E et al. J Mol Med (Berl) 2020; 98 (10):1431-1446 The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells.

实验应用 : FACS

种属反应 : Human

Aminuddin A et al. Sci Rep 2020; 10 (1):7885

实验应用 : WB

种属反应 : Human

Wang X et al. Stem Cell Res 2020; 42:101703 Generation of an iPSC line (HUSTi002-A) from fibroblasts of a patient with Sertoli cell-only syndrome carrying c.731_732delAT in PIWIL2 gene.

实验应用 : ICC/IF

种属反应 : Human

Chen YL et al. Cancers (Basel) 2019; 12 (1) ERK Activation Modulates Cancer Stemness and Motility of a Novel Mouse Oral Squamous Cell Carcinoma Cell Line.

Chien CH et al. J Biomed Sci 2019; 26 (1):77 Enrichment of superoxide dismutase 2 in glioblastoma confers to acquisition of temozolomide resistance that is associated with tumor-initiating cell subsets.

实验应用 : WB

种属反应 : Human

Huang TH et al. J Cell Mol Med 2019; 23 (12):8184-8195 In silico identification of thiostrepton as an inhibitor of cancer stem cell growth and an enhancer for chemotherapy in non-small-cell lung cancer.

实验应用 : WB

种属反应 : Human

Chen YH et al. Sci Rep 2019; 9 (1):9919 Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells.

实验应用 : ICC/IF

种属反应 : Human

Yang CE et al. Chem Biol Interact 2019; 302:28-35 the antipsychotic * suppresses yap signaling, stemness properties, and drug resistance in breast cancer cells.

实验应用 : WB

种属反应 : Human

Gupta N et al. Proc Natl Acad Sci U S A 2017; 114 (5):1033-1038 Stress granule-associated protein G3BP2 regulates breast tumor initiation.

实验应用 : WB

种属反应 : Human

Huang TT et al. Oncotarget 2015; 6 (20):18012-26 The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.

实验应用 : WB

种属反应 : Human

Wang AM et al. Oncotarget 2014; 5 (13):5002-16 Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.

实验应用 : WB

种属反应 : Human

Qin H et al. Cell 2014; 158 (2):449-61 Systematic Identification of Barriers to Human iPSC Generation.

实验应用 : WB

种属反应 : Human

SDS
PBS.pdf
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产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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Oct4抗体 [GT486]

目录号 GTX627419

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

Observed MW

阳性对照

预测种属反应

产品属性

形式

存储溶液

保存剂

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX627419 IP Image

GTX627419 WB Image

GTX627419 ICC/IF Image

GTX627419 IHC-P Image

GTX627419 IHC-P Image

GTX627419 WB Image

GTX627419 IHC-P Image

GTX627419 WB Image

GTX627419 FACS Image

GTX627419 WB Image

GTX627419 ICC/IF Image

GTX627419 WB Image

GTX627419 WB Image

GTX627419 WB Image

文献引用

Application Reference

产品评论

Oct4 antibody [GT486] 目录号 GTX627419

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