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Oct4抗体 [GT735]

KO/KD Overexpression
Anti-Oct4 antibody [GT735] used in Immunoprecipitation (IP). GTX627423
Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423
Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423
Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423
Anti-Oct4 antibody [GT735] used in Flow cytometry (FACS). GTX627423
Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423
Anti-Oct4 antibody [GT735] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627423

目录号 GTX627423

1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT735

同种型

 IgG1

实验应用

 WB, ICC/IF, FACS, IP

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Oct4 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

39 kDa. ( Note )

阳性对照

NT2D1 , OCT 3/4 transfected HeLa , human ESC

预测种属反应

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888015

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

细胞定位

Nucleus

功能与背景

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Oct4 antibody [GT735] used in Immunoprecipitation (IP). GTX627423

GTX627423 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627423) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627423) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423

GTX627423 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] (GTX627423) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423

GTX627423 WB Image

Overexpression

Sample (10 ug of whole cell lysate)
A: Non-transfected HeLa lysates
B: OCT 3/4 transfected HeLa lysates
10% SDS PAGE
GTX627423 diluted at 1:5000

Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423

GTX627423 WB Image

Oct4 antibody [GT735] detects Oct4 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
(GTX627423) dilution: 1:1000

Anti-Oct4 antibody [GT735] used in Flow cytometry (FACS). GTX627423

GTX627423 FACS Image

Oct4 antibody [GT735] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT735] dilution: 1:50

Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423

GTX627423 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX627423 diluted at 1:1000

Anti-Oct4 antibody [GT735] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627423

GTX627423 ICC/IF Image

Oct4 antibody detects Oct4 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX627423) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

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产品评论

Oct4 antibody [GT735] 目录号 GTX627423

Rating
( Average 4 based on 1 users reviews)
实验应用
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-Oct4 antibody [GT735] used in Flow cytometry (FACS). GTX627423
Date : Anonymous submitted on 11-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Black : Isotype control, Green : OCT4 antibody
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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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