应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
产品说明
Based on IEP, no cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.
形式
Liquid
存储溶液
TBS, 1% BSA, 10% heat-inactivated animal Serum
保存剂
0.1% ProClin 150
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原种属
Rabbit
免疫原
Purified Rabbit IgG, whole molecule
Target Isotype
IgG
纯化方式
Purified by antigen-affinity chromatography
纯度
> 95% by SDS-PAGE
偶联
Horseradish peroxidase(HRP)
RRID
AB_11164964
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
别名
anti-rabbit HRP polymer , IHC Polymer , antirabbit HRP polymer , anti rabbit HRP polymer
功能与背景
Immunohistochemistry (IHC) is the localization of antigens in tissue sections/cells using antibodies as specific reagents. Visualization of antibody signal is often significantly improved using the biotin-avidin amplification system. This system, however, adds significant time to experimental protocols and results in higher background due to the presence of endogenous biotin in tissue.
GeneTex’s One-Step anti-rabbit IgG (H+L) uses the power of next-generation Polymer technology to provide dramatic signal amplification without the need for biotin. This reagent has been shown to provide increased sensitivity and detection, while reducing the incubation time and required quantity of primary antibody. This reagent can be used to enhance staining of membranes, cytoplasmic and nuclear antigens. It provides the user with a rapid and easy to use IHC detection system.
Features and Benefits
‧ Lower background: Amplification without biotin avoids background from endogenous biotin
‧ Higher sensitivity than standard IHC detection
‧ Faster: Staining time reduced to 15 minutes!
‧ Compatible with either manual or automated systems
‧ Better results on difficult-to-work antibodies and nuclear staining
‧ Save cost due to further dilution of primary antibody
