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P-Cadherin抗体

Orthogonal
Anti-P-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX113648
Anti-P-Cadherin antibody used in Western Blot (WB). GTX113648

目录号 GTX113648

文献引用 1
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

91 kDa. ( Note )

预测种属反应

Rat, Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human P-cadherin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2036405

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

cadherin 3 , CDHP , HJMD , PCAD

细胞定位

Cell membrane

功能与背景

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-P-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX113648

GTX113648 IHC-P Image

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using P-cadherin(GTX113648) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-P-Cadherin antibody used in Western Blot (WB). GTX113648

GTX113648 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with P-Cadherin antibody (GTX113648) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

文献引用

Application Reference

Wen-Yu Yang et al. Bioeng Transl Med 2021; 6 (2) : e10212 Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes.
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产品评论

P-Cadherin antibody 目录号 GTX113648

Rating
( Average 4 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-P-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX113648
Date : Anonymous submitted on 13-Mar-2014
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : B6 mice E15.5,E18.5 embryo tissue
Blocking : Thermo Scientific SuperBlock Blocking Buffers, 25°C, 0.5Hr
Fixation : 4% Paraformaldehyde
Permeabilization /Antigen retrieval : Sodium citrate pH:6.0
Primary Antibody : 1:500, 4°C, 24Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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