Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX629076) diluted at 1:1000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody [GT635] (GTX629076) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: CD3EAP protein stained by CD3EAP antibody (GTX629076) diluted at 1:500.
Blue: Hoechst 33342 staining.

PAF49 antibody [GT635] detects CD3EAP protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
PAF49 antibody [GT635] (GTX629076) dilution: 1:1000