产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2 , A375 , A549 , H1299 , HCT116
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888094
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
CD3e molecule associated protein , ASE-1 , ASE1 , CAST , PAF49
细胞定位
Nucleus , nucleolus
功能与背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
数据库
研究领域
產品图片
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/templates/default/js/lightbox/images/loading.gif "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
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GTX629076 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody [GT635] (GTX629076) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629076 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody (GTX629076) diluted at 1:1000.
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GTX629076 ICC/IF Image
CD3EAP antibody detects CD3EAP protein at Nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: CD3EAP protein stained by CD3EAP antibody (GTX629076) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX629076 WB Image
PAF49 antibody [GT635] detects CD3EAP protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
PAF49 antibody [GT635] (GTX629076) dilution: 1:1000
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文献引用
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产品评论
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![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/small/GTX629076/GTX629076_41309_20170511_WB_shRNA_watermark_w_23061202_478.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/small/GTX629076/GTX629076_41309_20151224_WB_w_23061202_279.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629076](/upload/website/prouct_img/small/GTX629076/GTX629076_41309_IFA_w_23061202_705.webp "Anti-PAF49 antibody [GT635] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/small/GTX629076/GTX629076_41309_WB_w_23061202_718.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/normal/GTX629076/GTX629076_41309_20170511_WB_shRNA_watermark_w_23061202_478.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/normal/GTX629076/GTX629076_41309_20151224_WB_w_23061202_279.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
![Anti-PAF49 antibody [GT635] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629076](/upload/website/prouct_img/normal/GTX629076/GTX629076_41309_IFA_w_23061202_705.webp "Anti-PAF49 antibody [GT635] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629076")
![Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076](/upload/website/prouct_img/normal/GTX629076/GTX629076_41309_WB_w_23061202_718.webp "Anti-PAF49 antibody [GT635] used in Western Blot (WB). GTX629076")
