PARP抗体 [N2C1], Internal

Name: PARP抗体 [N2C1], Internal
Brand: GeneTex
SKU: GTX112864
Price: 4000.00 RMB
Rating: 4.5 (2 reviews)

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

Anti-PARP antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX112864

Anti-PARP antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX112864

Anti-PARP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112864

Anti-PARP antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112864

目录号 GTX112864

文献引用 10

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP, ChIP assay
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

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产品说明

摘要

PARP antibody (more specifically, PARP1 antibody) detects poly(ADP-ribose) polymerase (PARP)-1, which is involved in various biological mechanisms such as DNA repair, genomic stability, and programmed cell death. Preclinical and clinical studies showed that PARP inhibition is an effective anti-tumor modality, and thus PARP inhibitors have become essential components of various chemotherapeutic regimens against many malignancies.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

113 kDa. ( Note )

阳性对照

NIH-3T3 , PC-12 , 293T , A431 , HeLa , HepG2 , 293T

产品说明

IP/MS validation was supported by references (PMID:30377409)

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

1XPBS (pH7), 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11173565

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

细胞定位

Nucleus,Nucleolus

功能与背景

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

数据库

Gene ID: 142 PARP1
UniProt: P09874 PARP1

研究领域

產品图片

GTX112864 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112864 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112864 IP Image

PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 30 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal (GTX112864)
5 % SDS-PAGE
The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:3000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX112864 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
ChIP experiment and primer designs are based on BMC Mol Biol. 2006 Dec 22;7:48.

GTX112864 IHC-P Image

PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
PARP1 antibody [N2C1], Internal (GTX112864) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:10000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX112864 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.

GTX112864 IHC-P Image

PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112864 ICC/IF Image

PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112864 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112864 IHC-P Image

PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4) : 866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.

实验应用 : WB

种属反应 : Human

Kerrie-Ann McMahon et al. Elife 2021; 10 Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.

实验应用 : WB

种属反应 : Human

Jang M et al. Cancers (Basel) 2021; 13 (16) LNX1 Contributes to Cell Cycle Progression and Cisplatin Resistance.

实验应用 : WB

D繹rsam B et al. Proc Natl Acad Sci U S A 2018; 115 (17) : E4061-E4070 PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression.

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.

实验应用 : IP

种属反应 : Human

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我要回报文献

产品评论

PARP antibody [N2C1], Internal 目录号 GTX112864

Rating

( Average 4.5 based on 2 users reviews)

实验应用

Western Blot(WB)

( Average 4.5 based on 2 users reviews)

Date : Anonymous submitted on 31-Jan-2021

Score :

Application Tested : WB

Sample Species : Hu

Sample : iPSC

Amount used : 25 μg

Blocking : 5% milk, 25°C, 1Hr

Primary Antibody : 1:5000, 4°C, 16Hr

Date : Anonymous submitted on 24-Jul-2015

Score :

Application Tested : WB

Sample Species : Hu

Sample : H1299 cells

Amount used : 30μg

Blocking : 5% milk, 25°C, 1Hr

Primary Antibody : 1:3000, 4°C, 24Hr

我要评论产品

Application Reference

Seung-Hun Kim et al. Cancer Drug Resist 2021; 4 (4):866-880 The aqueous extract of brucea javanica reduces tumorigenicity of human lung cancer tumorspheres.

实验应用 : WB

种属反应 : Human

Kerrie-Ann McMahon et al. Elife 2021; 10 Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.

实验应用 : WB

种属反应 : Human

Jang M et al. Cancers (Basel) 2021; 13 (16) LNX1 Contributes to Cell Cycle Progression and Cisplatin Resistance.

实验应用 : WB

D繹rsam B et al. Proc Natl Acad Sci U S A 2018; 115 (17):E4061-E4070 PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression.

Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.

实验应用 : IP

种属反应 : Human

Kim SH et al. Biomedicine & Pharmacotherapy 2018; 99:997-1008 Teroxirone suppresses growth and motility of human hepatocellular carcinoma cells.

实验应用 : WB

种属反应 : Human

Chien T et al. Mol Psychiatry 2018; (Epub) GSK3β negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons.

实验应用 : WB

种属反应 : Rat

Wang JP et al. Oncol Lett 2017; 14 (3):3503-3509 Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.

Chen PH et al. Neuropharmacology 2016; (Epub) the chac1-inhibited notch3 pathway is involved in *-induced glioma cytotoxicity.

实验应用 : WB

种属反应 : Human

Buchkremer S et al. JND 2016; 3 (2):169-181 Sil1-Mutant Mice Elucidate Chaperone Function in Neurological Disorders.

实验应用 : IHC-P

种属反应 : Mouse

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
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PARP抗体 [N2C1], Internal

目录号 GTX112864

宿主

克隆

同种型

实验应用

种属反应

摘要

产品应用

应用说明

预期分子量

阳性对照

产品说明

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX112864 WB Image

GTX112864 WB Image

GTX112864 IP Image

GTX112864 ChIP assay Image

GTX112864 IHC-P Image

GTX112864 WB Image

GTX112864 WB Image

GTX112864 ChIP assay Image

GTX112864 IHC-P Image

GTX112864 ICC/IF Image

GTX112864 WB Image

GTX112864 WB Image

GTX112864 WB Image

GTX112864 IHC-P Image

文献引用

Application Reference

产品评论

PARP antibody [N2C1], Internal 目录号 GTX112864

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