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PARP 抗体 [N2C1], Internal

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX112864
Anti-PARP antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX112864
Anti-PARP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112864
Anti-PARP antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864
Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

目录号 GTX112864

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IP, ChIP assay

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥3590),
25 μl (¥1390)
产品说明

摘要

PARP antibody (more specifically, PARP1 antibody) detects poly(ADP-ribose) polymerase (PARP)-1, which is involved in various biological mechanisms such as DNA repair, genomic stability, and programmed cell death. Preclinical and clinical studies showed that PARP inhibition is an effective anti-tumor modality, and thus PARP inhibitors have become essential components of various chemotherapeutic regimens against many malignancies.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

113 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , HCT116 , HCT116 (30 μM cisplatin treatment for 24 hr) , NIH-3T3 , PC-12 , *H1299

特异性/灵敏度

IP/MS validation was supported by references (PMID:30377409)

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

细胞定位

Nucleus,Nucleolus

功能与背景

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX112864

GTX112864 IP Image

PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 30 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal (GTX112864)
5 % SDS-PAGE
The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:3000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-PARP antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX112864

GTX112864 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
ChIP experiment and primer designs are based on BMC Mol Biol. 2006 Dec 22;7:48.

Anti-PARP antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX112864

GTX112864 IHC-P Image

PARP1 antibody [N2C1], Internal detects PARP1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
PARP1 antibody [N2C1], Internal (GTX112864) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PARP antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112864

GTX112864 ICC/IF Image

PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:10000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PARP antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX112864

GTX112864 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP antibody [N2C1], Internal used in Western Blot (WB). GTX112864

GTX112864 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

产品评论

PARP antibody [N2C1], Internal 目录号 GTX112864

Rating ( Average 4 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 24-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1299 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 24Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 3590
¥ 1390
联系经销商
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