产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.5 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Quail
免疫原
The immunogen used to generate this antibody corresponds to Coturnix coturnix PAX3.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888531
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
paired box 3 , CDHS , HUP2 , WS1 , WS3
细胞定位
Nucleus
功能与背景
This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]
研究领域
產品图片
![Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463](/templates/default/js/lightbox/images/loading.gif "Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463")
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GTX635463 WB Image
U87-MG whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] (GTX635463) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635463 IHC-P Image
PAX3 antibody [HL160] detects PAX3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.PAX3 stained by PAX3 antibody [HL160] (GTX635463) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635463 IHC-P Image
PAX3 antibody [HL160] detects PAX3 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.PAX3 stained by PAX3 antibody [HL160] (GTX635463) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635463 IHC-P Image
PAX3 antibody [HL160] detects PAX3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: PAX3 stained by PAX3 antibody [HL160] (GTX635463) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635463 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] (GTX635463) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635463 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] (GTX635463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635463 IHC-P Image
PAX3 antibody [HL160] detects PAX3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.PAX3 stained by PAX3 antibody [HL160] (GTX635463) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463](/upload/website/prouct_img/small/GTX635463/GTX635463_44088_20210917_WB_Fraction_w_23061202_214.webp "Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463")
![Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463](/upload/website/prouct_img/small/GTX635463/GTX635463_44088_20221228_IHC-P_multiple_R_22122901_963.webp "Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463")
![Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463](/upload/website/prouct_img/small/GTX635463/GTX635463_44088_20221228_IHC-P_multiple_M_22122901_720.webp "Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463")
![Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463](/upload/website/prouct_img/small/GTX635463/GTX635463_43894_20221019_IHC-P_M_22102723_738.webp "Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463")
![Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463](/upload/website/prouct_img/small/GTX635463/GTX635463_44088_20220916_WB_B_22092119_559.webp "Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463")
![Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463](/upload/website/prouct_img/small/GTX635463/GTX635463_44088_20201008_WB_M_E10.5embryo_w_23061202_716.webp "Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463")
![Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463](/upload/website/prouct_img/small/GTX635463/GTX635463_44088_20210820_IHC-P_w_23061202_514.webp "Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463")
![Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463](/upload/website/prouct_img/normal/GTX635463/GTX635463_44088_20210917_WB_Fraction_w_23061202_214.webp "Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463")
![Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463](/upload/website/prouct_img/normal/GTX635463/GTX635463_44088_20221228_IHC-P_multiple_R_22122901_963.webp "Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463")
![Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463](/upload/website/prouct_img/normal/GTX635463/GTX635463_44088_20221228_IHC-P_multiple_M_22122901_720.webp "Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463")
![Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463](/upload/website/prouct_img/normal/GTX635463/GTX635463_43894_20221019_IHC-P_M_22102723_738.webp "Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463")
![Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463](/upload/website/prouct_img/normal/GTX635463/GTX635463_44088_20220916_WB_B_22092119_559.webp "Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463")
![Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463](/upload/website/prouct_img/normal/GTX635463/GTX635463_44088_20201008_WB_M_E10.5embryo_w_23061202_716.webp "Anti-PAX3 antibody [HL160] used in Western Blot (WB). GTX635463")
![Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463](/upload/website/prouct_img/normal/GTX635463/GTX635463_44088_20210820_IHC-P_w_23061202_514.webp "Anti-PAX3 antibody [HL160] used in IHC (Paraffin sections) (IHC-P). GTX635463")
