PAX8抗体

Name: PAX8抗体
Brand: GeneTex
SKU: GTX101583
Price: 4000.00 RMB

Anti-PAX8 antibody used in Immunoprecipitation (IP). GTX101583

Anti-PAX8 antibody used in ChIP assay (ChIP assay). GTX101583

Anti-PAX8 antibody used in IHC (Paraffin sections) (IHC-P). GTX101583

Anti-PAX8 antibody used in Western Blot (WB). GTX101583

Anti-PAX8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101583

Anti-PAX8 antibody used in Immunohistochemistry (IHC). GTX101583

目录号 GTX101583

文献引用 5

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
种属反应	Human, Mouse, Rat, Dog
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 PAX8 的产品

View product citations for antibody GTX101583 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:500-1:1000
ChIP assay	Assay dependent
IHC	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

48 kDa. ( Note )

阳性对照

293T nuclear extract , DDDDK-tagged PAX8-transfected 293T

预测种属反应

Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human PAX8. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1951123

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

paired box 8

细胞定位

Nucleus

功能与背景

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

数据库

Gene ID: 7849 PAX8
UniProt: Q06710 PAX8

研究领域

產品图片

GTX101583 IP Image

PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 μg of PAX8 antibody (GTX101583) 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 antibody (GTX101583) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101583 ChIP assay Image

PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of PAX8 antibody (GTX101583) The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.

GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at nucleolus by immunohistochemical analysis.
Sample: Paraffin-embedded DLD1 xenograft .
PAX8 antibody (GTX101583) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101583 WB Image

293T whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (GTX101583) diluted at 1:1000.

GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at cytosol and nucleus on rat thyroid gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thyroid gland.
PAX8 antibody (GTX101583) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101583 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (GTX101583) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PAX8 antibody (GTX101583) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101583 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8(GTX101583) antibody at 1:200 dilution.

GTX101583 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (GTX101583) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
PAX8 stained by PAX8 antibody (GTX101583) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min