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PCNA抗体

Anti-PCNA antibody used in Western Blot (WB). GTX100539
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100539
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100539
Anti-PCNA antibody used in Immunoprecipitation (IP). GTX100539
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in Western Blot (WB). GTX100539
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in Western Blot (WB). GTX100539

目录号 GTX100539

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr, IP, FISH, IHC

种属反应

Human, Mouse, Rat, Golden Syrian Hamster
产品包装
100 μl (¥3590),
25 μl (¥1390)
产品说明

摘要

PCNA antibody recognizes the proliferating cell nuclear antigen (PCNA), a cofactor of DNA polymerase delta with a predicted molecular weight of 29 kDa. PCNA is important for DNA synthesis, repair, and epigenetic modifications, and also serves as an organelle marker of the nucleus. PCNA antibody can be used for distinguishing between early, middle, and late S-phase of the cell cycle as well as grading of different neoplasms.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
FISH Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

29 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , NIH-3T3 , PC-12 , *A549 , *SiHa

产品说明

IP/MS validation was supported by references (PMID:30377373)

预测种属反应

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

1XPBS (pH7), 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1241163

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

proliferating cell nuclear antigen , ATLD2

细胞定位

Nucleus

功能与背景

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-PCNA antibody used in Western Blot (WB). GTX100539

GTX100539 WB Image

PCNA antibody detects PCNA protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
12% SDS-PAGE
PCNA antibody (GTX100539) dilution: 1:2500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
PCNA antibody (GTX100539) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100539

GTX100539 ICC/IF Image

PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon .
PCNA antibody (GTX100539) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100539

GTX100539 ICC/IF Image

PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PCNA antibody used in Immunoprecipitation (IP). GTX100539

GTX100539 IP Image

Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 μg of PCBP2 antibody (GTX100539).
Western blot analysis was performed using PCBP2 antibody (GTX100539).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PCNA antibody (GTX100539) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCNA antibody used in Western Blot (WB). GTX100539

GTX100539 WB Image

PCNA antibody detects PCNA protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
PCNA antibody (GTX100539) dilution: 1:2500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PCNA antibody (GTX100539) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PCNA antibody (GTX100539) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCNA antibody used in Western Blot (WB). GTX100539

GTX100539 WB Image

PCNA antibody detects PCNA protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX100539) diluted at 1:2500.

文献引用

产品评论

PCNA antibody 目录号 GTX100539

Rating ( Average 4.7 based on 13 users reviews)
实验应用
Western Blot(WB) ( Average 4.7 based on 9 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4.5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 08-Aug-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : A375 cells
Amount used : 30μg
Blocking : 5% non-fat milk, BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:2500, 4°C, > 12Hr
Date : Anonymous submitted on 07-Feb-2022
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : WAZ-2T +SA cell line derived fr BALB/c ms mammary tumor
Blocking : 1% BSA in PBS (pH7.6), 22°C, 1Hr
Fixation : 95% methnol
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS (pH7.6), 15min, 4°C
Primary Antibody : 1:100, 4°C, 16Hr
条带说明 :
Red: GTX100539 (Alxa Fluor 568)
Blue: DAPI
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27659793
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 28489070
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28533522
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28112237
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Toxins (Basel) in 2013. PMID: 24335753
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 27458161
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : normal colon tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Date : Anonymous submitted on 01-Oct-2019
Score :
Application Tested : WB
Sample Species : Golden Syrian Hamster
Sample : BHK21 cells
Amount used : 5 μg
Blocking : 2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 05-Jul-2018
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : PANC-1 cells
Blocking : PBS with 2% FBS and 0.4% Triton X-100, 25°C, 0.17Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : PBS with 2% FBS and 0.4% Triton X-100
Primary Antibody : 1:100, 4°C, 16Hr
Date : Anonymous submitted on 23-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Date : Anonymous submitted on 30-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells,SiHa cells,HeLa cells
Amount used : 10μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 3590
¥ 1390
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