产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human PD-L1 Intracellular domain, and it does not cross react with human PDL2 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide encompassing a sequence within the Intracellular domain of human PD-L1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909937
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1
细胞定位
Cell membrane
功能与背景
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
数据库
研究领域
產品图片
![Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033](/templates/default/js/lightbox/images/loading.gif "Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033")
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GTX636033 WB Image
Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636033 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636033 ICC/IF Image
PD-L1 antibody [HL1056] detects PD-L1 protein by immunofluorescent analysis.Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 10 min.Green: PD-L1 stained by PD-L1 antibody [HL1056] (GTX636033) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636033 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:1000 and competitor's antibody diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636033 WB Image
Untreated (–) and treated (+) MDA-MB-231 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL1056] (GTX636033) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033](/upload/website/prouct_img/small/GTX636033/GTX636033_44291_20220218_WB_KO_watermark_w_23061202_742.webp "Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033")
![Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033](/upload/website/prouct_img/small/GTX636033/GTX636033_44291_20220506_WB_B_w_23061202_894.webp "Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033")
![Anti-PD-L1 antibody [HL1056] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636033](/upload/website/prouct_img/small/GTX636033/GTX636033_44291_20221230_ICC_IF_22122901_653.webp "Anti-PD-L1 antibody [HL1056] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636033")
![Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033](/upload/website/prouct_img/small/GTX636033/GTX636033_T-44249_20210402_WB_competitor_watermark_w_23061202_783.webp "Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033")
![Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033](/upload/website/prouct_img/small/GTX636033/GTX636033_44291_20220225_WB_treatment_Tunicamycin_w_23061202_502.webp "Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033")
![Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033](/upload/website/prouct_img/normal/GTX636033/GTX636033_44291_20220218_WB_KO_watermark_w_23061202_742.webp "Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033")
![Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033](/upload/website/prouct_img/normal/GTX636033/GTX636033_44291_20220506_WB_B_w_23061202_894.webp "Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033")
![Anti-PD-L1 antibody [HL1056] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636033](/upload/website/prouct_img/normal/GTX636033/GTX636033_44291_20221230_ICC_IF_22122901_653.webp "Anti-PD-L1 antibody [HL1056] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636033")
![Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033](/upload/website/prouct_img/normal/GTX636033/GTX636033_T-44249_20210402_WB_competitor_watermark_w_23061202_783.webp "Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033")
![Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033](/upload/website/prouct_img/normal/GTX636033/GTX636033_44291_20220225_WB_treatment_Tunicamycin_w_23061202_502.webp "Anti-PD-L1 antibody [HL1056] used in Western Blot (WB). GTX636033")
