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PD-L1 抗体

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763
Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763
Anti-PD-L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104763
Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

目录号 GTX104763

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr

种属反应

Human
产品包装
100 μl (¥3590),
25 μl (¥1390)
产品说明

摘要

PD-L1 antibody recognizes programmed death-ligand 1 (PD-L1) protein, also known as B7 homolog 1 (B7-H1) and cluster of differentiation 274 (CD274) protein. PD-L1 is found on various cancer cells and transduces immunosuppressive signals by binding to the programmed cell death protein 1 (PD-1) on effector T cells, thereby diminishing immune system attacks on malignant cells. Blocking the interaction between PD-L1 and PD-1 with PD-L1 antibodies is generating great clinical interest as a mode of immunotherapy against a number of cancers.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

33 kDa. ( Note )

Observed MW

50 kDa. The observed M.W. is based on the following publication. PMID: 23674495

阳性对照

A431 , MDA-MB-231 , MDA-MB-231 (24μg/ml Tunicamycin treatment for 16 hr) , MDA-MB-231 , A549 (100 ng/mL IFN-gamma treatment for 48 hr) , A431 (100 ng/mL IFN-gamma treatment for 48 hr)

产品属性

形式

Liquid

存储溶液

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1

细胞定位

Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Endomembrane system; Single-pass type I membrane protein

功能与背景

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

数据库

研究领域

產品图片

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:600 and competitor's antibody (CST#13684) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:1200 and competitor's antibody (CST#13684) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:600 and with DDDDK tag antibody (GTX115043) diluted at 1:3000 to detect DDDDK-tagged PD-L2. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763

GTX104763 IHC-P Image

PD-L1 antibody detects PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
PD-L1 antibody (GTX104763) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763

GTX104763 IHC-P Image

PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody (GTX104763) diluted at 1:2000 and competitor's antibody (CST#13684) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763

GTX104763 IHC-P Image

PD-L1 antibody detects PD-L1 protein at cell membrane in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody (GTX104763) diluted at 1:1000, and competitor's antibody diluted at 1:50. Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-PD-L1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104763

GTX104763 IHC-P Image

PD-L1 antibody detects PD-L1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
PD-L1 stained by PD-L1 antibody (GTX104763) diluted at 1:4000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD-L1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104763

GTX104763 ICC/IF Image

PD-L1 antibody detects PD-L1 protein by immunofluorescent analysis.
Sample: MDA-MB-231 (left) and HeLa (right) cells were fixed in ice-cold MeOH for 5 min.
Green: PD-L1 stained by PD-L1 antibody (GTX104763) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-PD-L1 antibody used in Western Blot (WB). GTX104763

GTX104763 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

文献引用

产品评论

PD-L1 antibody 目录号 GTX104763

Rating ( Average 3 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 22-May-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SK-LMS-1 cells
Amount used : 30 μg
Blocking : 3%BSA, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 3590
¥ 1390
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