产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
NCI-H929 , HL-60 , DDDDK-tagged PD1-transfected 293T
预测种属反应
Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human PD1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885778
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
programmed cell death 1 , CD279 , PD-1 , PD1 , SLEB2 , hPD-1 , hPD-l , hSLE1
细胞定位
Membrane; Single-pass type I membrane protein
功能与背景
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX128435 IHC-P Image
PD1 antibody detects PD1 protein at cell membrane of inflammatory cells in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX128435 WB Image
PD-1 antibody detects PD-1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PD-1 antibody (GTX128435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX128435 WB Image
PD1 antibody detects PDCD1 protein by western blot analysis.
A. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
PD1 antibody (GTX128435) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX128435 IHC-P Image
PD1 antibody detects PD1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse lymph node.PD1 stained by PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX128435 IHC-P Image
PD1 antibody detects PD1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.PD1 stained by PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX128435 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD1 antibody (GTX128435) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
文献引用
产品评论
目前没有使用 PD1 antibody (GTX128435) 的产品评论被收录。快发表第一篇产品评论吧！
