产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
K562 , PC-12
预测种属反应
Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.79 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human PDGF Receptor alpha. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1951151
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
platelet derived growth factor receptor alpha , CD140A , PDGFR-2 , PDGFR2
细胞定位
Membrane; Single-pass type I membrane protein
功能与背景
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903](/templates/default/js/lightbox/images/loading.gif "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903")
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GTX107903 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:500.
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GTX107903 WB Image
Sample (30 ug of whole cell lysate)
A: K562
5% SDS PAGE
GTX107903 diluted at 1:1000
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GTX107903 ICC/IF Image
PDGF Receptor alpha antibody [N2C2], Internal detects PDGF Receptor alpha protein at cytoplasm by immunofluorescent analysis.Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PDGF Receptor alpha stained by PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:500.Red: phalloidin, a cytoskeleton marker diluted at 1:200.Blue: Hoechst 33342 staining.
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GTX107903 WB Image
Human tissue extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX107903 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal (GTX107903) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
产品评论
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![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903](/upload/website/prouct_img/small/GTX107903/GTX107903_39988_20160121_WB_R_w_23060120_934.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903")
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903](/upload/website/prouct_img/small/GTX107903/GTX107903_39988_WB_w_23060120_760.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903")
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107903](/upload/website/prouct_img/small/GTX107903/GTX107903_39988_20191025_ICC_IF_w_23060120_673.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107903")
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903](/upload/website/prouct_img/small/GTX107903/GTX107903_39988_20220617_WB_skeletalmuscle_22062121_572.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903")
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903](/upload/website/prouct_img/small/GTX107903/GTX107903_39988_20230616_WB_M_Differentiated_23062019_816.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903")
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903](/upload/website/prouct_img/normal/GTX107903/GTX107903_39988_20160121_WB_R_w_23060120_934.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903")
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903](/upload/website/prouct_img/normal/GTX107903/GTX107903_39988_WB_w_23060120_760.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903")
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107903](/upload/website/prouct_img/normal/GTX107903/GTX107903_39988_20191025_ICC_IF_w_23060120_673.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107903")
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903](/upload/website/prouct_img/normal/GTX107903/GTX107903_39988_20220617_WB_skeletalmuscle_22062121_572.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903")
![Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903](/upload/website/prouct_img/normal/GTX107903/GTX107903_39988_20230616_WB_M_Differentiated_23062019_816.webp "Anti-PDGF Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX107903")
