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PER2抗体

KO/KD Orthogonal Overexpression
Anti-PER2 antibody used in Western Blot (WB). GTX129688
Anti-PER2 antibody used in Western Blot (WB). GTX129688
Anti-PER2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129688
Anti-PER2 antibody used in Western Blot (WB). GTX129688
Anti-PER2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129688
Anti-PER2 antibody used in Western Blot (WB). GTX129688

目录号 GTX129688

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 PER2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

137 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human PER2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886072

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

period circadian regulator 2 , FASPS , FASPS1

细胞定位

Nucleus , Cytoplasm

功能与背景

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-PER2 antibody used in Western Blot (WB). GTX129688

GTX129688 WB Image

Orthogonal

Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with PER2 antibody (GTX129688) and BMAL1 antibody [N1N3] (GTX105060) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 27834218

Anti-PER2 antibody used in Western Blot (WB). GTX129688

GTX129688 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PER2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129688

GTX129688 ICC/IF Image

PER2 antibody detects PER2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PER2 stained by PER2 antibody (GTX129688) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PER2 antibody used in Western Blot (WB). GTX129688

GTX129688 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER2 antibody (GTX129688) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PER2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129688

GTX129688 IHC-P Image

PER2 antibody detects PER2 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: PER2 protein stained by PER2 antibody (GTX129688) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PER2 antibody used in Western Blot (WB). GTX129688

GTX129688 WB Image

Orthogonal

Untreated and treated 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

YY Chiou et al. J Biol Chem 2024; : 105637 Circadian modulation of glucose utilization via CRY1-mediated repression of Pdk1 expression
实验应用 : WB
Ding M et al. Front Physiol 2022; 13 : 1024038 Acute hypoxia induced dysregulation of clock-controlled ovary functions.
Yongkang Zhen et al. Front Immunol 2022; 13 : 848248 Normal Light-Dark and Short-Light Cycles Regulate Intestinal Inflammation, Circulating Short-chain Fatty Acids and Gut Microbiota in Period2 Gene Knockout Mice.
实验应用 : WB
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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