产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human PFKFB4 protein, and it does not cross react with human PFKFB1, PFKFB2, and PFKFB3 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human PFKFB4.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
功能与背景
The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
数据库
研究领域
產品图片
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/templates/default/js/lightbox/images/loading.gif "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
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GTX638208 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638208 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638208 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638208 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638208 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638208 IHC-P Image
PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.PFKFB4 stained by PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638208 IHC-P Image
PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat spleen.PFKFB4 stained by PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638208 ICC/IF Image
PFKFB4 antibody [HL2204] detects PFKFB4 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.Green: PFKFB4 stained by PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
目前没有使用 PFKFB4 antibody [HL2204] (GTX638208) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 PFKFB4 antibody [HL2204] (GTX638208) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/small/GTX638208/GTX638208_45117_20230728_WB_23073119_424.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/small/GTX638208/GTX638208_45117_20230818_WB_M_23082201_206.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/small/GTX638208/GTX638208_45117_20230818_WB_R_23082201_574.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/small/GTX638208/GTX638208_T-44942_20230526_WB_multiple_B_23053001_374.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/small/GTX638208/GTX638208_T-44942_20230602_WB_TPM_watermark_23060622_403.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208](/upload/website/prouct_img/small/GTX638208/GTX638208_T-44942_20230816_IHC-P_M_23090619_908.webp "Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208](/upload/website/prouct_img/small/GTX638208/GTX638208_T-44942_20230816_IHC-P_R_23090619_336.webp "Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638208](/upload/website/prouct_img/small/GTX638208/GTX638208_45117_20240301_ICC_IF_24031823_953.webp "Anti-PFKFB4 antibody [HL2204] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/normal/GTX638208/GTX638208_45117_20230728_WB_23073119_424.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/normal/GTX638208/GTX638208_45117_20230818_WB_M_23082201_206.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/normal/GTX638208/GTX638208_45117_20230818_WB_R_23082201_574.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/normal/GTX638208/GTX638208_T-44942_20230526_WB_multiple_B_23053001_374.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208](/upload/website/prouct_img/normal/GTX638208/GTX638208_T-44942_20230602_WB_TPM_watermark_23060622_403.webp "Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208](/upload/website/prouct_img/normal/GTX638208/GTX638208_T-44942_20230816_IHC-P_M_23090619_908.webp "Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208](/upload/website/prouct_img/normal/GTX638208/GTX638208_T-44942_20230816_IHC-P_R_23090619_336.webp "Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208")
![Anti-PFKFB4 antibody [HL2204] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638208](/upload/website/prouct_img/normal/GTX638208/GTX638208_45117_20240301_ICC_IF_24031823_953.webp "Anti-PFKFB4 antibody [HL2204] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638208")
