产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , Molt-4 , Raji , mouse heart , mouse lung
预测种属反应
Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human PGD. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1951203
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
phosphogluconate dehydrogenase , 6PGD
功能与背景
6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703](/templates/default/js/lightbox/images/loading.gif "Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703")
|
GTX101703 ICC/IF Image
PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGD protein stained by PGD antibody [N1N3] (GTX101703) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX101703 WB Image
PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (GTX101703) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101703 ICC/IF Image
PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PGD protein stained by PGD antibody [N1N3] (GTX101703) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX101703 WB Image
Sample(30 μg whole cell lysate)
A:293T
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
10% SDS PAGE
GTX101703 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101703 IHC-P Image
PGD antibody [N1N3] detects PGD protein at cytoplasm on A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
PGD antibody [N1N3] (GTX101703) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101703 WB Image
PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (GTX101703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101703 WB Image
The data was published in the journal PLoS Biol in 2010.PMID: 21049082
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文献引用
产品评论
目前没有使用 PGD antibody [N1N3] (GTX101703) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703](/upload/website/prouct_img/small/GTX101703/GTX101703_39946_20150410_IFA_w_23060100_834.webp "Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703")
![Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703](/upload/website/prouct_img/small/GTX101703/GTX101703_39946_20151029_WB_M_lung_w_23060100_290.webp "Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703")
![Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703](/upload/website/prouct_img/small/GTX101703/GTX101703_39946_IFA_w_23060100_425.webp "Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703")
![Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703](/upload/website/prouct_img/small/GTX101703/GTX101703_WB_w_23060100_791.webp "Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703")
![Anti-PGD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101703](/upload/website/prouct_img/small/GTX101703/GTX101703_IHC_w_23060100_870.webp "Anti-PGD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101703")
![Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703](/upload/website/prouct_img/small/GTX101703/GTX101703_39946_20151015_WB_M_heart_w_23060100_278.webp "Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703")
![Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703](/upload/website/prouct_img/small/GTX101703/GTX101703_20240411_citeab_WB_1431_24041501_517.webp "Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703")
![Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703](/upload/website/prouct_img/normal/GTX101703/GTX101703_39946_20150410_IFA_w_23060100_834.webp "Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703")
![Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703](/upload/website/prouct_img/normal/GTX101703/GTX101703_39946_20151029_WB_M_lung_w_23060100_290.webp "Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703")
![Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703](/upload/website/prouct_img/normal/GTX101703/GTX101703_39946_IFA_w_23060100_425.webp "Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703")
![Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703](/upload/website/prouct_img/normal/GTX101703/GTX101703_WB_w_23060100_791.webp "Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703")
![Anti-PGD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101703](/upload/website/prouct_img/normal/GTX101703/GTX101703_IHC_w_23060100_870.webp "Anti-PGD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101703")
![Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703](/upload/website/prouct_img/normal/GTX101703/GTX101703_39946_20151015_WB_M_heart_w_23060100_278.webp "Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703")
![Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703](/upload/website/prouct_img/normal/GTX101703/GTX101703_20240411_citeab_WB_1431_24041501_517.webp "Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703")
