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PI3 kinase p150抗体 [C1C3]

KO/KD Overexpression
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Immunoprecipitation (IP). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

目录号 GTX108953

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IP

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

153 kDa. ( Note )

阳性对照

Molt-4 , Raji , 293T

预测种属反应

Rat, Bovine, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human PI3 kinase p150. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1951244

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

phosphoinositide-3-kinase regulatory subunit 4 , VPS15 , p150

功能与背景

Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.

数据库

研究领域

產品图片

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] (GTX108953) diluted at 1:500.

Anti-PI3 kinase p150 antibody [C1C3] used in Immunoprecipitation (IP). GTX108953

GTX108953 IP Image

Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody [C1C3] (GTX108953).
Western blot analysis was performed using PI3-Kinase p150 antibody [C1C3] (GTX108953).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
7.5% SDS PAGE
GTX108953 diluted at 1:1000

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

KO/KD

The data was published in the journal Nat Commun in 2015. PMID: 26387534

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

KO/KD

The data was published in the journal Nat Commun in 2015. PMID: 26387534

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

Overexpression

The data was published in the journal Nat Commun in 2019. PMID: 30952952

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

The data was published in the journal Nat Commun in 2015. PMID: 26387534

文献引用

Application Reference

Alkhoury C et al. Nat Cell Biol 2023; 25 (7) : 975-988 Class 3 PI3K coactivates the circadian clock to promote rhythmic de novo purine synthesis.
Iershov A et al. Nat Commun 2019; 10 (1) : 1566 The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα.
实验应用 : WB
种属反应 : Mouse
Nemazanyy I et al. Nat Commun 2015; 6 : 8283 class iii pi3k regulates organismal glucose homeostasis by providing negative feedback on hepatic * signalling.
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Glycerol.pdf
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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