产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Jurkat , mouse brain , rat brain , zebrafish liver , whole zebrafish
预测种属反应
Rabbit, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.25 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PKC alpha. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886274
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
protein kinase C alpha , AAG6 , PKC-alpha , PKCA , PKCI+/- , PKCalpha , PRKACA
功能与背景
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX130453 IHC-Fr Image
PKC alpha antibody detects PKC alpha protein in the rod bipolar cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX130453 ICC/IF Image
PKC alpha antibody detects PKC alpha protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX130453 WB Image
Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (GI: gastrointestinal)
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GTX130453 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:2000.
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GTX130453 IHC-Fr Image
PKC alpha antibody detects PKC alpha protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Red: PKC alpha protein stained by PKC alpha antibody (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX130453 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody (GTX130453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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