产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human PKC gamma protein, and it does not cross react with human PKC alpha, PKC beta 1, PKC beta 2, PKC delta, PKC epsilon, PKC zeta, PKC eta, and PKC theta protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragmemt of human PKC gamma.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
protein kinase C gamma , PKC-gamma , PKCC , PKCG , PKCI(3) , PKCgamma , SCA14
细胞定位
Cytoplasm,Cytoplasm, perinuclear region,Cell membrane,Synapse, synaptosome,Cell projection, dendrite
功能与背景
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
数据库
研究领域
產品图片
![Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271](/templates/default/js/lightbox/images/loading.gif "Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271")
|
GTX638271 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (GTX638271) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX638271 WB Image
Human brain (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (GTX638271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX638271 WB Image
PKC proteins (10 ng) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (GTX638271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX638271 IHC-P Image
PKC gamma antibody [HL2226] detects PKC gamma protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.PKC gamma stained by PKC gamma antibody [HL2226] (GTX638271) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX638271 IHC-P Image
PKC gamma antibody [HL2226] detects PKC gamma protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.PKC gamma stained by PKC gamma antibody [HL2226] (GTX638271) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
文献引用
目前没有使用 PKC gamma antibody [HL2226] (GTX638271) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 PKC gamma antibody [HL2226] (GTX638271) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271](/upload/website/prouct_img/small/GTX638271/GTX638271_45040_20230512_WB_M_R_23051702_478.webp "Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271")
![Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271](/upload/website/prouct_img/small/GTX638271/GTX638271_45040_20230512_WB_brain_23051702_106.webp "Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271")
![Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271](/upload/website/prouct_img/small/GTX638271/GTX638271_T-44956_20230317_WB_Ag_23032022_758.webp "Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271")
![Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271](/upload/website/prouct_img/small/GTX638271/GTX638271_T-44956_20230325_IHC-P_M_23032819_984.webp "Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271")
![Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271](/upload/website/prouct_img/small/GTX638271/GTX638271_T-44956_20230325_IHC-P_multiple_R_23032819_229.webp "Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271")
![Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271](/upload/website/prouct_img/normal/GTX638271/GTX638271_45040_20230512_WB_M_R_23051702_478.webp "Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271")
![Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271](/upload/website/prouct_img/normal/GTX638271/GTX638271_45040_20230512_WB_brain_23051702_106.webp "Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271")
![Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271](/upload/website/prouct_img/normal/GTX638271/GTX638271_T-44956_20230317_WB_Ag_23032022_758.webp "Anti-PKC gamma antibody [HL2226] used in Western Blot (WB). GTX638271")
![Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271](/upload/website/prouct_img/normal/GTX638271/GTX638271_T-44956_20230325_IHC-P_M_23032819_984.webp "Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271")
![Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271](/upload/website/prouct_img/normal/GTX638271/GTX638271_T-44956_20230325_IHC-P_multiple_R_23032819_229.webp "Anti-PKC gamma antibody [HL2226] used in IHC (Paraffin sections) (IHC-P). GTX638271")
