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PPAR alpha抗体 [3B6/PPAR]

Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779
Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX22779
Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779
Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22779
Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779

目录号 GTX22779

宿主

Mouse

克隆

Monoclonal

克隆编号

3B6/PPAR

同种型

IgG2b

实验应用

WB, ICC/IF, FACS, IP, ChIP assay, Gel supershift assays

种属反应

Human, Mouse, Rat, Rabbit, Bovine
产品包装
100 μg (¥4800)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 2-5 μg/ml
ICC/IF 2μg/ml
FACS 1μg per 10⁶ cells
IP Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

52 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Purified recombinant PPAR alpha protein.

纯化方式

Purified IgG

偶联

Unconjugated

RRID

AB_11166646

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR

细胞定位

Nucleus

功能与背景

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779

GTX22779 FACS Image

FACS analysis of Hela cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PPAR alpha antibody [3B6/PPAR] used in Western Blot (WB). GTX22779

GTX22779 WB Image

WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX22779 PPAR alpha antibody [3B6/PPAR].
Dilution : 1:1000 (Hela and Jurkat) and 1:500 (NIH-3T3)

Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779

GTX22779 FACS Image

FACS analysis of 3T3 cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PPAR alpha antibody [3B6/PPAR] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22779

GTX22779 ICC/IF Image

ICC/IF analysis of HCT116 cells using GTX22779 PPAR alpha antibody [3B6/PPAR]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-PPAR alpha antibody [3B6/PPAR] used in Flow cytometry (FACS). GTX22779

GTX22779 FACS Image

FACS analysis of Jurkat cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

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SDS
PBS.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 4800
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